| 抗HBs单克隆抗体Fv段基因的克隆及其表达 |
| |
| 引用本文： | 张春英,冯百芳,殷珊,仝文斌,陶其敏.抗HBs单克隆抗体Fv段基因的克隆及其表达[J].北京大学学报(医学版),1995(1). |
| |
| 作者姓名： | 张春英 冯百芳 殷珊 仝文斌 陶其敏 |
| |
| 作者单位： | 北京医科大学肝病研究所 |
| |
| 摘 要： | 用聚合酶链反应（ＰＣＲ）获得抗－ＨＢｓ单克隆抗体Ｆｖ段轻、重链基因，经连接后形成一条单链含轻、重链的Ｆｖ基因（ＳｃＦｖ），并在重组－噬菌体－抗体系统（ＲＰＡＳ）中获得表达，用酶联免疫吸附法（ＥＬＩＳＡ）证明表达的Ｆｖ段具有特异性抗原结合活性。
|
| 关 键 词： | 肝炎抗体，抗体，单克隆，基因表达，聚合酶链反应 |
CLONING AND EXPRESSION OF Fv GENE OF ANTI-HBs McAB Hepatology |
| |
| Institute Zhang Chunying,Feng Baifang,et al.CLONING AND EXPRESSION OF Fv GENE OF ANTI-HBs McAB Hepatology[J].Journal of Peking University:Health Sciences,1995(1). |
| |
| Authors: | Institute Zhang Chunying Feng Baifang |
| |
| Abstract: | mmunoglobin
Fv genes of anti-HBs McAb were amplified by PCR from a hybridoma cell
line secreting anti-HBs monoclonal antibody.The heavy chain and light
chain were linked to become single chain Fv gene fragment and were
expressed by recombinant phage antibody system(RPAS).The specific
antigen binding activity of the Fvexpressed was demonstrated by
enzyme linked immunoassay. |
| |
| Keywords: | Headings Hepatitis antibodies Antibodie monoclonal Gene expression Polymerase chain reaction |
| 本文献已被 CNKI 等数据库收录！ |
