川芎嗪对大鼠肝脏缺血再灌注损伤的保护作用

目的：研究川芎嗪对大鼠肝脏缺血再灌注损伤的保护作用及其机制。方法：SD大鼠随机分为5组：空白对照组、假手术组、缺血再灌注组、生理盐水组和川芎嗪组。其中空白对照组大鼠直接处死;假手术组开腹后60 min关闭腹腔;缺血再灌注组阻断70%肝血流60 min,再灌注4 h;生理盐水组予腹腔内注射生理盐水8 ml/kg,30 min后阻断70%肝血流60 min,再灌注4 h;川芎嗪组予腹腔内注射川芎嗪80 mg/kg,30 min后阻断70%肝血流60 min,再灌注4 h。速率法测血清丙氨酸氨基转移酶（alanine aminotransferase,ALT）和天冬氨酸氨基转移酶（aspartate aminotransferase,AST）活性,酶联免疫吸附法（ELISA）测血白介素-1（IL-1）、白介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）水平。苏木素-伊红染色观察肝脏病理改变。结果：川芎嗪组血清ALT、AST、IL-1和TNF-α水平均比缺血再灌注组和生理盐水组明显降低（P〈0.05）,而IL-10则明显高于缺血再灌注组和生理盐水组（P〈0.05）。病理结果显示,川芎嗪组大鼠肝细胞损伤较缺血再灌注组和生理盐水组为轻。结论：川芎嗪对大鼠肝脏缺血再灌注损伤具有保护作用,其机制可能与抑制炎性细胞因子生成及促进抗炎细胞因子表达有关。

Protective effect of ligustrazine against ischemia-reperfusion injury in rat liver

Objective:To investigate the protective effect of ligustrazine against ischemia-reperfusion (IR) injury in rat liver and its mechanism. Methods: Thirty-eight male SD rats were randomly divided into control group (n=6), sham-operation group (n=6), ischemia-reperfusion group (n=10), normal saline group (n=6) and ligustrazine group (n=10).The rats in control group were sacrificed without any treatment. The rat in sham-operation group underwent a midline laparotomy for 60 min. The rats in ischemia-reperfusion group received a 60-min occlusion of 70% hepatic blood inflow, followed by 4 h reperfusion. The rats in normal saline group or in ligustrazine group received normal saline (8 ml/kg) or ligustrazine (80 mg/kg) intraperitoneally 30 min prior to occlusion of 70% hepatic blood inflow.After 4 h reperfusion, rats were sacrified. Serum activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by rate method and the liver histological changes were examined after hematoxylin-eosin (HE) staining.Serum concentrations of tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1) and interleukin-10 (IL-10) were detected by enzyme linked immunosorbent assay (ELISA). Results: Serum levels of ALT and AST,serum concentrations of IL-1 and TNF-α were markedly decreased (P<0.05), while IL-10 concentration was obviously increased (P<0.05) in ligustrazine group compared with the ischemia-reperfusion group and normal saline group. The injury of hepatocytes was ameliorated in ligustrazine group compared with the ischemia-reperfusion group and normal saline group. Conclusion:Ligustrazine can reduce the ischemia-reperfusion injury of rat liver by inhibiting the expression of proinflammation cytokines and promoting anti-inflammation cytokine IL-10 expression.