Urine protein concentration estimation for biomarker discovery |
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| Authors: | Hiten D. Mistry Kate Bramham Andrew J. Weston Malcolm A. Ward Andrew J. Thompson Lucy C. Chappell |
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| Affiliation: | 1. Department of Biochemistry and Molecular Biology, Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Republic of Korea;2. Division of Nutrition and Metabolism Research Group, Korea Food Research Institute, Gyeonggi-do, Republic of Korea;3. Department of Obstetrics, Gynecology & Reproductive Biology, Michigan State University College of Human Medicine, MI, USA;4. Fertility Center of CHA General Hospital, CHA Research Institute, CHA University, Seoul, Republic of Korea;5. Department of Biomedical Sciences, University of Ulsan College of Medicine, Seoul, Republic of Korea |
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| Abstract: | Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia). BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation. |
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| Keywords: | Protein concentration assays Proteomics Urine |
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