去酰基化胃饥饿素对高脂饮食诱导C57BL/6J小鼠的抗疲劳作用

目的探究去酰基化胃饥饿素(DAG)对高脂饮食(HFD)诱导的肥胖小鼠的抗疲劳作用。方法36只6周龄C57BL/6小鼠随机分为对照(NC)组、高脂(HFD)组、HFD+DAG组(每组各12只),分别予以标准饲料(NC组)、高脂饲料(HFD组、HFD+DAG组)喂养,并给予每日腹腔注射生理盐水(NC组,HFD组)和DAG(HFD+DAG组)。喂养16 w后行力竭测试和负重游泳实验,随后处死小鼠取血检测血清乳酸(LA)、乳酸脱氢酶(LDH)、尿素氮(BUN)水平;留取肝脏和股四头肌测定肝糖原和肌糖原水平。结果与NC组相比,HFD组体重上升,腓肠肌和股四头肌指数下降,力竭运动距离和游泳力竭时间均缩短,血清BUN、LA水平升高,血清LDH、肝糖原、肌糖原含量下降,差异有统计学意义(P<0.05,P<0.01)。与HFD组相比,HFD+DAG组力竭运动距离和游泳力竭时间延长,血清BUN、LA水平下降,血清LDH、肝糖原、肌糖原含量升高,差异有统计学意义(P<0.05,P<0.01)。结论长期应用DAG有助于提高肥胖小鼠的运动耐力与抗疲劳能力。

Anti-fatigue effect of des-acyl ghrelin(DAG)on obese mice induced by high fat diet

Objective To investigate the anti-fatigue effect of des-acyl Ghrelin(DAG)on obese mice induced by high fat diet(HFD).Methods Thirty-six male C57BL/6J mice were randomly divided into normal control group(NC),high-fat-diet group(HFD),and HFD+DAG group(12 mice in each group),and were fed with standard diet(NC),high fat diet(HFD,HFD+DAG).Saline(NC,HFD),DAG(HFD+DAG)were given intraperitoneally once a day.At the end of 16 weeks,an exhaustion test and a weight-bearing swimming test were performed.Levels of serum lactic acid(LA),lactic dehydrogenase(LDH),urea nitrogen(BUN),as well as liver glycogen and muscle glycogen were detected.Results Compared with the NC group,the mice in the HFD group showed a significant increase in body weight.HFD decreased the gastrocnemius and quadriceps index,as well as exhausted exercise distance and swimming exhaustion time.HFD also increased serum BUN and LA levels.Levels of serum LDH,liver glycogen and skeletal muscle glycogen were decreased.DAG showed significant effects on anti-fatigue activity by increasing the serum LDH levels and decreasing the LA and BUN levels.The levels of liver and skeletal muscle glycogen were also significantly increased.Conclusions Long-term application of DAG could improve exercise performance and anti-fatigue ability of obese mice.