苏黄止咳胶囊对大鼠慢阻肺模型NF-ΚB p65、TLR4及IAP1表达的影响

目的:探讨苏黄止咳胶囊对大鼠慢阻肺模型的治疗作用及作用机制.方法:60只大鼠随机分为空白对照组、模型组、苏黄止咳胶囊低剂量组(0.36g/kg)、苏黄止咳胶囊中剂量组(0.72g/kg)、苏黄止咳胶囊高剂量组(1.44g/kg)、氨茶碱片组(0.01g/kg),除空白组外其余大鼠于实验第1天和第15天滴鼻注入0.2ml...

Effect of Suhuang Zhike Capsule on Expression of NF-KB p65,TLR4 and IAP1 in Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease

Objective:To study the therapeutic effect and mechanism of Suhuang Zhike Capsule on rat model of chronic obstructive pulmonary disease.Methods:Sixty rats were randomly divided into control group,model group,low dose group of Suhuang Zhike Capsule(0.36g/kg),middle dose group of Suhuang Zhike Capsule(0.72g/kg),high dose group of Suhuang Zhike Capsule(1.44 g/kg),aminophylline tablets group(0.01 g/kg).All rats except the blank group were intranasally injected with 0.2 ml of LPS(1 g/L)solution on the first and the 15th day of the experiment.After the model was treated for 4 weeks,the maximum expiratory flow(PEP)and the forced expiratory volume(FEV1)of the rats were measured using an animal respiratory function tester one hour after the last administration.The pathological changes of the lungs of each group were observed by HE staining and immunohistochemical staining.Results:The middle and high dose groups of Suhuang Zhike Capsule significantly increased the maximum expiratory flow(PEP)and forced expiratory volume(FEV1)in rats with chronic obstructive pulmonary disease.The results of HE staining showed that the inflammation grade of Suhuang Zhike capsule in middle and high dose group was significantly decreased,and the expression of NF KB p65(nuclear factor kappa-B p65)and TLR4(Toll like receptors 4)in lung of rats in middle and high dose group was significantly decreased;the expression of IAP1(inhibitor of apoptotic protein 1)in the each group of Suhuang Zhike Capsule was significantly decreased.Conclusion:Suhuang Zhike Capsule may reduce the expression of NF-KB p65,IAP1 and TLR4 in lungs of rats with chronic obstructive pulmonary disease by improving lung function,thereby alleviating lung injury in rats with chronic obstructive pulmonary disease.