巨细胞病毒肝炎婴儿外周血IL-33、sST2表达及意义

目的 探讨白细胞介素-33(IL-33)、可溶性致癌抑制因子2(sST2)在婴儿巨细胞病毒(HCMV)肝炎中的表达及意义。方法 选取HCMV感染婴儿40例,其中肝炎组20例,其他疾病组20例;另选择同期健康婴儿20例作为对照组。采用ELISA法检测三组婴儿血浆IL-33、sST2、IFN-γ、IL-4浓度,采用流式细胞术检测外周血Th1、Th2细胞比例。结果HCMV阳性肝炎组和HCMV阳性其他疾病组患儿的血浆IL-33、sST2、IFN-γ、IL-4浓度及Th1、Th2细胞比例均高于对照组,肝炎组高于其他疾病组。肝炎组和其他疾病组患儿IFN-γ/IL-4及Th1/Th2均低于对照组,肝炎组低于其他疾病组,差异均有统计学意义(P<0.05)。HCMV肝炎组血浆IL-33浓度与sST2、IL-4浓度及Th2细胞比例呈显著正相关(P<0.05),与IFN-γ/IL-4、Th1/Th2呈显著负相关(P<0.05)。结论 HCMV肝炎婴儿存在Th1/Th2失衡,IL-33可能参与HCMV感染所致Th2优势应答和Th1/Th2失衡。

Expression and significance of IL-33 and sST2 in peripheral blood of cytomegalovirus hepatitis in infants

Objective To explore the expression and significance of interleukin-33 (IL-33) and soluble suppression of tumorigenicity 2 (sST2) in human cytomegalovirus (HCMV) hepatitis in infants. Methods Forty infants with HCMV infection were selected, including 20 in hepatitis group and 20 in other diseases group, and 20 healthy infants in the same period were selected as the control group. The plasma levels of IL-33, sST2, IFN-γ and IL-4 were measured by ELISA, and the ratio of Th1 and Th2 cells in peripheral blood was measured by flow cytometry. Results The plasma levels of IL- 33, sST2, IFN-γ, IL-4 and the proportion of Th1 and Th2 cells in HCMV-positive hepatitis group and other diseases group were higher than those in the control group, and they were higher in hepatitis group than in other diseases group. The levels of IFN-γ/IL-4 and Th1/Th2 in hepatitis group and other diseases group were lower than those in the control group, and they were lower in hepatitis group than in other diseases group. The differences were statistically significant (P<0.05). Plasma IL-33 concentration in HCMV-positive hepatitis group was positively correlated with sST2 and IL-4 concentrations and the proportion of Th2 cell (P<0.05), while it was negatively correlated with the IFN-γ/IL-4 and Th1/Th2 (P<0.05). Conclusion Th1/Th2 imbalance exists in infants with HCMV hepatitis. IL-33 may be involved in Th2 dominant response and Th1/Th2 imbalance caused by HCMV infection.