外源性一氧化氮对旋毛虫感染小鼠抗氧化能力的影响

目的探讨外源性NO供体亚硝基铁氰化钠(SNP)对旋毛虫病小鼠肝脏、外周血中抗氧化酶活性及脂质氧化的影响。方法采用消化法分离旋毛虫肌幼虫,感染BALB/c小鼠,400条/只。42 d后,收集感染鼠及正常小鼠外周血、肝脏。设A组(感染鼠肝脏匀浆物+SNP)、B组(正常鼠肝脏匀浆物+SNP)、C组(感染鼠外周血+SNP)、D组(正常鼠外周血+SNP)。每组内设5个SNP反应终浓度,分别为0(空白对照)、2、5、10、30μmol/L,37℃反应30 min,测定各组超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)及脂质氧化(MDA)含量。结果与正常鼠相比,旋毛虫感染鼠肝脏、外周血中SOD、CAT、GSH-Px活性上升,MDA含量增加,差异均有统计学意义(P均0.05);在A、B组中,10、30μmol/L SNP作用下SOD、CAT、GSH-Px活性较空白对照降低(P均0.05);与2~30μmol/L SNP反应后,MDA含量较空白对照明显升高(P均0.05)。在C、D组中,30μmol/LSNP作用下SOD、CAT、GSH-Px活性显著下降,MDA浓度升高,与空白对照比较差异均有统计学意义(P均0.01)。在2~30μmol/L浓度区间,SNP浓度与旋毛虫感染鼠肝脏、外周血中SOD、CAT、GSH-Px活性呈负相关(r肝SOD=-0.901,r肝CAT=-0.802,r肝GSH-Px=-0.847,r血SOD=-0.899,r血CAT=-0.685,r血GSH-Px=-0.635,P均0.05),与MDA含量呈正相关(r肝MDA=0.697,r血MDA=0.764,P均0.05)。结论旋毛虫感染可致小鼠肝脏、外周血中活性氧自由基产生增加,SOD、CAT、GSH-Px活性升高;外源性NO可降低旋毛虫感染小鼠SOD、CAT、GSH-Px活性,抑制小鼠的抗氧化能力。

Effect of exogenous nitric oxide on antioxidants from mice infected with Trichinella spiralis

Objective To study the effect of exogenous nitric oxide donor sodium nitroprusside （SNP） on antioxidant enzymes activities and lipid peroxidation of mice infected with Trichinella spiralis. Methods BALB/c mice were infected with T. spiralis separated by the digestion method. Forty?two days post?infection， the peripheral blood and hepatic tissue from the infected or normal mice were collected. Then 4 groups were set： liver homogenate from infected mice + SNP （Group A）， liver homogenate from normal mice + SNP （Group B）， peripheral blood from infected mice + SNP （Group C）， and peripheral blood from normal mice + SNP （Group D）. The final concentrations of SNP in each group were set as 0 （blank control）， 2， 5， 10 μmol/L and 30 μmol/L， respectively. After reacting with SNP at 37 ℃ for 30 min， the superoxide dismutase （SOD）， glutathione peroxidase （GSH?Px）， catalase （CAT） activities， and malondialdehyde （MDA） concentration were examined and compared. Results The levels of SOD， CAT， GSH?Px and MDA concentration in the liver and the blood from the mice infected with T. spiralis were significantly higher than those of the normal ones （all P < 0.05）. When reacted with 10 μmol/L and 30 μmol/L SNP， the SOD， GSH?Px， and CAT activities in Group A and B decreased significantly （all P < 0.05）， while the liver MDA concentration reacted with 2-30 μmol/L SNP increased obviously （all P < 0.05）. As reacted with 30 μmol/L SNP， the activities of blood SOD， GSH?Px， and CAT in Group C and D decreased， while the MDA concentration in blood still increased （all P < 0.01）. When the SNP concentration was in the range of 2-30 μmol/L， there were a negative correlation between the SNP concentrations and SOD， GSH?Px， and CAT activities， as well as a positive correlation with the MDA concentration in the liver and blood from the mice infected with T. spiralis （all P < 0.05）. Conclusions T. spiralis infection could cause oxidative damage to mice， and increase SOD， GSH?Px， and CAT activities. Nitric oxide released from SNP can decrease antioxidase activities， and inhibit the antioxidant capacity of mice infected with T. spiralis.