重离子辐照对人舌鳞癌CAL27细胞的生物学效应及其分子机制

目的 研究重离子12C6+离子辐照对人舌鳞癌CAL27细胞的生物学效应以及分子机制。方法 应用不同剂量重离子束（12C6+）对体外培养的人舌癌CAL27细胞进行辐照，采用MTT、Transwell、流式细胞术、划痕实验、克隆实验观察重离子束（12C6+）对CAL27细胞迁移和侵袭、凋亡和周期的影响，Western blot法观察重离子束（12C6+）对CAL27细胞增殖的影响。结果 （1）重离子辐照对CAL27细胞的增殖抑制作用与剂量-时间成正相关；（2）和空白对照组比较，在相同时间点，不同剂量的重离子束（12C6+）对细胞中P53、P65和VEGF的表达影响明显，随着重离子束（12C6+）辐照剂量增大，细胞中P53、P65和VEGF的表达明显降低（P<0.05）。结论 重离子辐照能抑制CAL27细胞的增殖，且具有剂量和时间效应。

Biological Effect and Molecular Mechanism of Heavy Ion Irradiation on Human Tongue Squamous Cell Carcinoma CAL27 Cells

Objective The biological effect and molecular mechanism of heavy ion 12C6+ ion irradiation on human tongue squamous cell carcinoma CAL27 cells. Methods We applied different doses of heavy ion beam (12C6+) to irradiate in vitro cultured human tongue cancer CAL27 cells. We adopted MTT, Transwell migration and invasion, flow cytometry, wound scratch assay and colony formation assay to observe the effect of heavy ion beam (12C6+) on CAL27 cells migration, invasion, apoptosis and cycle. Western blot was used to observe the effect tof heavy ion beam (12C6+) on CAL27 cells proliferation. Results The inhibitory effect of heavy ion irradiation on CAL27 cells proliferation was positively correlated with dose and time. Compared with blank control group, at the same time point, the effect of different doses of heavy ion beam (12C6+) on P53, P65 and VEGF expression was evidently. With the increasing irradiation dose of heavy ion beam (12C6+), the P53, P65 and VEGF expression were decreased significantly (P<0.05). Conclusion Heavy ion irradiation inhibits the proliferation of CAL27 cells in a dose- and time-dependent manner.