| miR-130b调控RAB34的表达在胰腺癌发生发展中的作用 |
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| 引用本文： | 何峰,陈曦,程方雄,闵小春,曹忠厚,丁锋. miR-130b调控RAB34的表达在胰腺癌发生发展中的作用[J]. 肿瘤防治研究, 2018, 45(3): 144-147. DOI: 10.3971/j.issn.1000-8578.2018.17.0960 |
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| 作者姓名： | 何峰 陈曦 程方雄 闵小春 曹忠厚 丁锋 |
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| 作者单位： | 1. 430077 武汉，华中科技大学同济医学院附属普爱医院检验科；2. 430064 武汉，武汉市江汉区疾病预防控制中心检验科；3. 430019 武汉，武汉市长江航务管理局疾病预防控制中心 |
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| 基金项目： | 武汉市卫计委科研基金（WX13C15） |
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| 摘 要： | 目的 探讨miR-130b调控Ras相关蛋白34（Ras-related proteins in brain 34, RAB34）在胰腺癌中的表达。方法 收集22例胰腺癌及其癌旁组织标本，采用免疫组织化学、Western blot和qRT-PCR法检测RAB34的表达；qRT-PCR法检测miR-130b表达；细胞转染法检测miR-130b调控RAB34表达，双荧光素酶报告基因检测系统验证miR-130b结合RAB34的3’UTR的靶位点。结果 与癌旁组织对比，RAB34在胰腺癌组织中显著高表达，且miR-130b显著低表达；转染miR-130b mimics可抑制RAB34的表达；RAB34是miR-130b作用的靶基因。结论 RAB34是胰腺癌的分子标志物，miR-130b可调控其表达。
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| 关 键 词： | 胰腺癌 RAB34 miR-130b |
| 收稿时间： | 2017-08-07 |
Effect of miR-130b Targeting RAB34 Expression in Occurrence and Development of Pancreatic Cancer |
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| HE Feng,CHEN Xi,CHENG Fangxiong,MIN Xiaochun,CAO Zhonghou,DING Feng. Effect of miR-130b Targeting RAB34 Expression in Occurrence and Development of Pancreatic Cancer[J]. Cancer Research on Prevention and Treatment, 2018, 45(3): 144-147. DOI: 10.3971/j.issn.1000-8578.2018.17.0960 |
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| Authors: | HE Feng CHEN Xi CHENG Fangxiong MIN Xiaochun CAO Zhonghou DING Feng |
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| Affiliation: | 1. Department of Laboratory, Puai Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, China; 2. Clinical Laboratory, Jianghan Center for Disease Prevention and Control in Wuhan, Wuhan 430064, China; 3. Disease Prevention and Control of Changjiang River Administration in Wuhan, Wuhan 430019, China |
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| Abstract: | Objective To investigate the expression of Ras-related proteins in brain 34 (RAB34) regulated by miR-130b in pancreatic cancer. Methods We collected 22 paired specimens of primary pancreatic cancer and adjacent normal tissues specimens. Immunohistochemistry, Western blot and qRT-PCR were employed to detect the expression level of RAB34. Meanwhile, the expression of miR-130b was detected by qRT-PCR. Cell transfection method was used to discover the regulation of miR-130b on RAB34 gene. The luciferase reporter assay was applied to identify whether RAB34 was directly targeted by miR-130b. Results Compared with adjacent tissues specimens, miR-130b was significantly downregulated and its target gene RAB34 was upregulated in pancreatic cancer tissues. Overexpression of miR-130b remarkably inhibited the expression of RAB34. Moreover, the dual luciferase assay revealed that RAB34 was directly targeted by miR-130b. Conclusion RAB34 might have a considerable potential in prognosis identification of pancreatic cancer and be regulated by miR-130b. |
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| Keywords: | Pancreatic cancer RAB34 miR-130b R735.9 |
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