顺铂联合补中益气汤对A549/DDP荷瘤裸鼠移植瘤survivin蛋白表达的影响

目的 探讨顺铂联合补中益气汤对A549/DDP荷瘤裸鼠移植瘤survivin蛋白表达的影响。方法 30只BALB/c-nu裸小鼠随机分为5组，A：荷瘤对照组、B：顺铂组、C：顺铂联合低剂量补中益气汤组、D：顺铂联合中剂量补中益气汤组、E：顺铂联合高剂量补中益气汤组。25 d后处死全部裸小鼠。测量移植瘤体积、重量并计算药物抑瘤率；Real-time PCR法检测移植瘤组织survivin mRNA的表达；Western blot法检测移植瘤组织survivin蛋白的表达。结果 顺铂联合低、中、高剂量组均较荷瘤对照组、顺铂组移植瘤体积显著缩小，重量显著减轻，差异有统计学意义；顺铂抑瘤率随着补中益气汤的剂量增加而增大；顺铂组、顺铂联合低剂量组较荷瘤对照组移植瘤组织中survivin的基因和蛋白表达上调，而顺铂联合中、高剂量组较荷瘤对照组survivin的基因和蛋白表达下调，差异均有统计学意义；顺铂联合低、中、高剂量补中益气汤组较顺铂组移植瘤组织中survivin的基因和蛋白表达均下调，差异均有统计学意义。结论 顺铂联合补中益气汤可通过下调survivin的表达改善A549/DDP荷瘤裸鼠移植瘤对顺铂的耐药性。

Effect of Cisplatin Combined with Buzhong Yiqi Decoction on survivin Expression in Transplantation Tumor of Nude Mice with A549/DDP Cells

Objective To explore effect of cisplatin combined with Buzhong Yiqi decoction on survivin expression in transplantation tumor of nude mice with A549/DDP cells. Methods Thirty BALB/c-nu mice were randomly divided into five groups: A group (tumor control group), B group (cisplatin group), C group (cisplatin with low dose of Buzhong Yiqi decoction), D group (cisplatin with medium dose of Buzhong Yiqi decoction), E group (cisplatin with high dose of Buzhong Yiqi decoction). DDP or DDP and Buzhong Yiqi decoction were treated from 9 to 33 day, lastly the mice were executed. The volume and weight of transplantation tumors were measured; tumor inhibitory rate was calculated. Real-time PCR was used to determine the expression of survivin mRNA; Western blot was adopted to detect the expression of survivin protein. Results The volume and weight of transplantation tumors in C, D and E groups were significantly smaller than those in A and B groups. Tumor inhibitory rates were increased with the increasing dose of Buzhong Yiqi decoction. Compared with A group, the survivin gene and protein levels of transplantation tumors in B and C groups were higher, however, the results were lower in D and E groups. The survivin gene and protein expressions of transplantation tumors in C, D and E groups were significantly down-regulated, compared with B group. Conclusion Cisplatin combined with Buzhong Yiqi decoction could improve cisplatin resistance of transplantation tumor of nude mice with A549/DDP cells through down-regulating the gene and protein expression of survivin.