2015年江西省"三甲医院"寄生虫病检验技能竞赛成绩分析

目的 了解江西省综合性三甲医院检验人员寄生虫病检测能力。方法 全省20家综合性三甲医院组队参赛，每队2名选手。竞赛分为笔试和技能操作两部分（各100分）。笔试内容涉及寄生虫生活史、免疫学基础、检测技术、病原诊断等；技能操作包括厚、薄血涂片及改良加藤厚涂片的制片和镜检阅片。结果 共40名选手参赛，总成绩平均分为（97.3±22.4）分，及格率为15.0%；其中笔试平均成绩为（56.6±12.8）分，及格率为52.5%；技能操作平均成绩为（40.8±12.4）分，及格率为5.0%。笔试成绩中，土源性线虫生活史得分率最高（90.0%），食源性寄生虫病防治基础知识得分率最低（31.5%）。选手血涂片制作、镜检及格率均高于加藤片，差异均有统计学意义（P均 < 0.05）；间日疟原虫、恶性疟原虫、卵形疟原虫和阴性片的镜检检出率依次为38.8%、45.0%、35.0%和25.0%；蠕虫卵检出率以鞭虫卵最高（87.5%），曼氏迭宫绦虫卵最低（2.5%）。省级医院选手加藤片制作和镜检得分均高于市级医院 （P均 < 0.05）；教学医院选手加藤片制片成绩高于非教学医院（P < 0.05）。结论 江西三甲医院检验专业人员寄生虫病检测技能水平难以满足当前寄生虫病诊断的需要，应引起卫生主管部门和医院的高度重视。

Analysis of results of technique competition for parasitic disease diagnosis in Class A tertiary hospitals in Jiangxi Province, 2015

Objective To understand the capability of parasitic disease diagnosis among professionals from the clinic laboratory of Class A tertiary hospitals in Jiangxi Province. Methods The teams that took part in the competition were formed from 20 Class A tertiary hospitals， with 2 contestants per team. The competition contents included written examination and skill operation. The written examination involved the life cycle of parasites， immunological basis， detecting techniques， etiological diagnosis etc.， and the skill operation involved making and dying thin and thick blood smears， making Kato?Katz’s fecal thick smears， as well as microscopic examinations of smears. Results A total of 40 participants took part in the competition. Their total average score was 97.3±22.4 with the pass rate of 15.0%， in which the mean score of theoretical knowledge was 56.6±12.8 with the pass rate of 52.5%， and the mean score of skill operation was 40.8±12.4 with the pass rate of 5.0%. In the written examination， the scoring rate of the life cycle of soil?transmitted helminths was the highest （90.0%）， and the rate of the basic knowledge of food?borne parasites was the lowest （31.5%）. Both the pass rates of blood smear making and examination were higher than those of Kato?Katz’s thick smears， and the differences were statistically significant （ both P < 0.05）. The detection rates of Plasmodium vivax， Plasmodium falciparum， Plasmodium ovale and negative slides of the contestants were 38.8%， 45.0%， 35.0% and 25.0% respectively. As to the microscopic examination of helminth eggs， the detection rate of Trichuris trichiura egg was the highest （87.5%）， and the rate of the Sparganum mansoni egg was the lowest （2.5%）. The scores of Kato?Katz’s thick smear making and examination of the contestants from the provincial level hospitals were higher than those from the city level hospitals （both P < 0.05）， and the score of those from teaching hospitals in Kato?Katz’s thick smear making was higher than that of those from non?teaching hospitals （P < 0.05）. Conclusions The technique level of professionals from the clinic laboratory of the Class A tertiary hospitals cannot meet the needs of the diagnosis of parasitic diseases in Jiangxi Province， which should arouse the attention of the health authorities and hospitals.