子宫颈鳞状细胞癌组织中LncRNA HCG11和miR-590-3p的表达及其与预后的关系

 目的 探讨LncRNA HCG11和miR-590-3p在子宫颈鳞状细胞癌组织及配对的癌旁组织中的表达情况，以及二者表达水平与患者临床资料和预后的相关性。方法 收集58例子宫颈鳞状细胞癌和配对的癌旁正常组织标本，运用qRT-PCR法检测58对子宫颈鳞状细胞癌组织及相应的癌旁组织中的LncRNA HCG11和miR-590-3p的表达，分析与宫颈鳞状细胞癌临床病理和预后的相关性，并评价其临床意义。结果 荧光定量PCR显示宫颈鳞状细胞癌组织中LncRNA HCG11的表达比癌旁组织明显下调（P<0.05），miR-590-3p的表达比癌旁组织明显上调（P<0.05）；二者在核酸表达水平上呈负相关（r=-0.642, P=0.000）。 LncRNA HCG11和miR-590-3p的表达均与宫颈癌淋巴结转移、组织大小及临床分期之间存在显著的相关性，且与预后相关（P<0.05）。Cox回归多因素分析发现LncRNA HCG11和miR-590-3p分别可作为宫颈鳞状细胞癌患者独立的预后因子。结论 LncRNA HCG11和miR-590-3p可作为宫颈鳞状细胞癌独立的预后标志物和潜在的治疗靶点，LncRNA HCG11可能通过调控miR-590-3p的表达量从而发挥抑癌基因的作用。

Expression of LncRNA HCG11 and miR-590-3p in Squamous Carcinoma of Cervix and Their Relationship with Prognosis

Objective To investigate the expression of long non-coding RNA (LncRNA) HCG11 and miR-590-3p in squamous carcinoma of cervix and paired adjacent tissues, and their correlation with patients' clinical data and prognosis. Methods Specimens of cervical cancer tissues and matched non-tumor para-neoplastic tissues were collected from 58 patients who underwent surgery in The Second People’s Hospital of Nanyang. Quantitative real-time PCR was used to examine the expression of LncRNA HCG11 and miR-590-3p, the correlation between them was be analyzed, and we also analyzed their correlation with clinicopathological features and prognosis. Results LncRNA HCG11 expression was significantly downregulated in squamous carcinoma of cervix tissues as compared with adjacent non-tumor tissues(P<0.05); the expression of miR-590-3p was significantly up-regulated in squamous carcinoma of cervix tissues as compared with the adjacent non-tumor tissues(P<0.05); their expression levels of nucleic acid showed a negative correlation (r=0.642, P=0.000). LncRNA HCG11 and miR-590-3p expression were significantly correlated with FIGO stage, lymph node metastasis, tissue size of tumor and prognosis(P<0.05). Multivariate analysis suggested that LncRNA HCG11 or miR-590-3p expression served as independent predictors for overall survival respectively. Conclusion LncRNA HCG11 and miR-590-3p may be potential prognostic markers and therapeutic targets for squamous carcinoma of cervix. LncRNA HCG11 may play the role of tumor suppressor by means of regulating the expression of miR-590-3p.