少突胶质细胞瘤中染色体1p/19q联合缺失与MGMT基因启动子甲基化的相关性

目的 研究少突胶质细胞瘤染色体1p/19q联合缺失与MGMT基因启动子甲基化的相关性。方法 选取少突胶质细胞瘤35例、间变型少突胶质细胞瘤32例标本作为实验组，星形细胞瘤20例及瘤旁正常脑组织标本20例作为对照组，荧光原位杂交方法检测染色体1p/19q联合缺失情况，巢式甲基化特异性PCR技术检测MGMT基因启动子甲基化情况。结果 少突胶质细胞瘤和间变型少突胶质细胞瘤1p/19q联合缺失率分别为85.71%、75.00%，均显著高于星形细胞瘤及正常脑组织（P<0.05）；少突胶质细胞瘤、间变型少突胶质细胞瘤、星形细胞瘤的MGMT基因启动子甲基化率均显著高于正常脑组织（P<0.05），但三者组间比较差异无统计学意义（P>0.05）；少突胶质细胞瘤1p/19q联合缺失与MGMT基因启动子甲基化呈正相关性，并且均与患者3年生存率相关（P<0.05）。结论 少突胶质细胞瘤中1p/19q联合缺失与MGMT基因启动子甲基化状态密切相关，联合检测有助于提高临床病理诊断的精确性，更好地评估预后。

Correlation Between Codeletion of Chromosome 1p/19q and MGMT Promoter Methylation in Oligodendrogliomas

Objective To explore the relationship between chromosome 1p/19q codeletion and MGMT promoter methylation in oligodendrogliomas. Methods We collected 35 cases of oligodendroglioma and 32 cases of anaplastic oligodendroglioma tissue specimens as the experimental group, and 20 cases of astrocytomas and 20 cases of normal brain tissue specimens as the control group. Fluorescence in situ hybridization (FISH) was used to detect the chromosome 1p/19q codeletion, and the methylation status of MGMT promoter was analyzed by methylation-specific PCR (MSP). Results The frequencies of the 1p/19q codeletion in oligodendrogliomas and anaplastic oligodendrogliomas were 85.71% and 75% respectively, which were significantly higher than those in astrocytomas and normal brain tissues(P<0.05). The frequencies of the MGMT promoter methylation in oligodendrogliomas, anaplastic oligodendrogliomas and astrocytomas were significantly higher than those in normal brain tissues(P<0.05), but there was no statistical difference among each other(P>0.05). The Spearman correlation analysis showed that the 1p/19q codeletion was positively correlated with the MGMT promoter methylation in oligodendrogliomas. Both 1p/19q codeletion and MGMT promoter methylation were correlated with the 3-year survival rate(P<0.05). Conclusion 1p/19q codeletion is closely linked with MGMT promoter methylation in oligodendrogliomas, therefore, the combined detection of these two biomarkers may be helpful for diagnosis and evaluating the prognosis.