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| X线辐射剂量对结直肠癌细胞中microRNA-221和p57kip2表达的影响 | |
| 引用本文： | 孙 凯,张晓槟,邓海军,钟育波,雷尚通,区文弢,吴承堂.X线辐射剂量对结直肠癌细胞中microRNA-221和p57kip2表达的影响[J].肿瘤防治研究,2013,40(10):921-924. |
| 作者姓名： | 孙 凯 张晓槟 邓海军 钟育波 雷尚通 区文弢 吴承堂 |
| 作者单位： | 1. 510515广州，南方医科大学南方医院普通外科；2.南方医科大学附属佛山医院普外三科 |
| 基金项目： | 国家自然科学基金青年科学基金资助项目 (81101896);广东省科技计划项目资助课题（2012B031800141）； 广东省医学科研基金资助项目（B2012221）；广东高校优秀青年 创新人才培养计划资助项目（2012LYM0035）；佛山市卫生局 医学科研基金资助项目（2012079）；南方医科大学南方医院院 长基金资助项目（2011B008） |
| 摘 要： | 目的 探讨不同剂量X线辐射对人结直肠癌Caco2细胞系中microRNA-221（miR-221）和p57kip2表达的影响。方法 常规培养Caco2细胞系并分为5组，分别给予不同剂量X线（0、2、4、6和8 Gy）照射，24 h后提取细胞总RNA和蛋白质，应用real-time Q-PCR检测细胞中miR-221和p57kip2 mRNA的表达水平，Western blot检测p57kip2蛋白的表达变化。结果 Caco2细胞系在不同照射剂量下，miR-221表达水平随着照射剂量的增加而增加，而p57kip2蛋白表达水平则随着照射剂量的增加而逐步降低，且呈剂量依赖效应，两者差异均具有统计学意义（P<0.05）。结论 放射线辐射剂量可影响结直肠癌细胞中miR-221/ p57kip2调控通路，抑制miR-221表达可能会提高结直肠癌细胞的放射敏感度。 |
| 关 键 词： | 结直肠癌 microRNA-221 p57kip2 辐射剂量 放射敏感度 |
| 收稿时间： | 2012-12-13 |
Effects of X-ray Dose on Expression of microRNA-221 and p57kip2 in Human Colorectal Carcinoma Cells |
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| SUN Kai,ZHANG Xiaobin,DENG Haijun,ZHONG Yubo,LEI Shangtong,OU Wentao,WU Chengtang.Effects of X-ray Dose on Expression of microRNA-221 and p57kip2 in Human Colorectal Carcinoma Cells[J].Cancer Research on Prevention and Treatment,2013,40(10):921-924. | |
| Authors: | SUN Kai ZHANG Xiaobin DENG Haijun ZHONG Yubo LEI Shangtong OU Wentao WU Chengtang |
| Institution: | 1.Department of General Surgery, Nangfang Hospital, Southern Medical University, Guangzhou 510515,China;2.The Third Department of General Surgery, Foshan Hospital, Southern Medical University(*:Tied for The First Author;Present:The Second Department of General Surgery,The Affiliated Nanhai Hospital, Southern Medical University, Foshan 528000,China) Corresponding |
| Abstract: | Objective To investigate the effects of X-ray dose on the expression of microRNA-221(miR-221) and p57kip2 in human colorectal carcinoma (CRC) cells. Methods Conventionally cultured Caco2 cells were divided into 5 groups with different doses of X-ray (0, 2, 4,6 and 8 Gy) respectively. The total RNA and protein of the cells were extracted after irradiation for 24 h,The expression level of miR-221 and p57kip2 mRNA were detected by real-time Q-PCR. The expression of p57kip2 in cells was detected by Western blot analysis. Results The radiation dose of X-ray had signifi cant effect on the expression of miR-221 and p57kip2 protein in human Caco2 cells in a dose-dependent manner. The miR-221 expression level improved gradually with the increase of irradiation dose while the p57kip2 protein expression level reduced gradually (P<0.05). Conclusion Radiation dose could affect the miR-221/p57kip2 regulation pathways in CRC cells. Suppressed miR-221 expression may enhance the radiosensitivity of CRC cells. |
| Keywords: | Colorectal carcinoma microRNA-221 p57kip2 Radiation dose Radiosensitivity R735 3 |
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