旋毛虫成虫排泄分泌蛋白抗CLP诱导的小鼠脓毒症的观察 |
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引用本文: | 杨小迪,贺文欣,方强,宋迪,吴琦,王小莉,李楠,齐琦,万勇坤,张慧,周瑞,陈兴智,刘牧林,李徽徽,褚亮.旋毛虫成虫排泄分泌蛋白抗CLP诱导的小鼠脓毒症的观察[J].中国血吸虫病防治杂志,2016,28(3):293-296,322. |
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作者姓名: | 杨小迪 贺文欣 方强 宋迪 吴琦 王小莉 李楠 齐琦 万勇坤 张慧 周瑞 陈兴智 刘牧林 李徽徽 褚亮 |
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作者单位: | 1 蚌埠医学院病原生物学教研室, 安徽省感染与免疫重点实验室 (蚌埠 23303); 2 蚌埠医学院组织学与胚胎学教研室; 3 蚌埠医学
院病理学教研室; 4蚌埠医学院第一附属医院; 5 蚌埠医学院第二附属医院 |
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基金项目: | 国家自然科学基金 (81441120); 安徽高校科研创新平台团队项目 (2016?40); 安徽省自然科学基金 (1508085QH158); 安徽省高校
优秀青年人才支持计划重点项目(gxyqZD2016159); 安徽省高等学校自然科学研究重点项目(KJ2016A708、 KJ2016A489、
KJ2016A869) |
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摘 要: | 目的 目的 探讨旋毛虫成虫排泄分泌蛋白 (AES) 对盲肠结扎穿孔术 (CLP) 诱导的小鼠脓毒症的抑制作用。方法 方法
将48只BALB/c小鼠随机分为3组, 即假手术对照组 (PBS + sham组, A组)、 脓毒症组 (PBS + CLP组, B组)、 AES蛋白治疗
组 (AES + CLP组, C组)。A组和B组于CLP术后经腹腔注射200 μl PBS, C组术后经腹腔注射含25 μg AES蛋白的等量
PBS。每组随机抽取8只, 建模后观察各组小鼠的一般情况及96 h生存率; 每组其余8只小鼠于建模后12 h取肝、 肺、 肾,
HE染色观察病理改变; 以酶联免疫吸附试验 (ELISA) 检测小鼠血清中TNF?α、 IL?1β、 IL?6、 IL?10及TGF?β细胞因子水平
的变化。结果 结果 3组小鼠96 h生存率差异有统计学意义 (χ2
= 21.16, P < 0.05); 与A组 (100%) 相比, B组小鼠生存率 (0)
降低 (P < 0.05), 且肺、 肝及肾脏损伤明显加重; 经AES治疗后, C组存活率 (75%) 明显增加 (P < 0.05), 小鼠肺脏、 肝脏及
肾脏病理损伤亦显著缓解。3组小鼠血清中的促炎因子TNF?α (F = 27.11, P < 0.05)、 IL?1β (F = 18.75, P < 0.05) 及IL?6
(F = 100.93, P < 0.05) 水平差异均有统计学意义; B组小鼠的促炎因子水平均高于A组 (P均< 0.05); 经AES治疗后, C组
小鼠上述3种促炎因子水平较B组均显著下降 (P均< 0.05)。3组小鼠血清中的调节性细胞因子IL?10 (F = 10.88, P <
0.05) 及TGF?β (F = 11.37, P < 0.05) 水平差异亦有统计学意义; 经AES治疗后, C组小鼠IL?10及TGF?β分泌水平较B组亦
明显增加 (P < 0.05)。结论 结论 旋毛虫AES对CLP诱导的BALB/c小鼠脓毒症有显著的缓解作用。
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关 键 词: | 旋毛虫 盲肠结扎穿孔术 排泄分泌蛋白 脓毒症 小鼠 促炎因子 抑炎因子 |
Effect of excretory/secretory protein of Trichinella spiralis adult worm on CLP-induced sepsis in mice |
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Institution: | 1 Department of Microbiology and Parasitology,Anhui Key Laboratory of Infection and Immunity,Bengbu Medical College,
Bengbu 233030,China;2 Department of Histology and Embryology,Bengbu Medical College,China;3 Department of Patholo?
gy,Bengbu Medical College,China;4 First Affiliated Hospital of Bengbu Medical College,China;5 The Second Affiliated Hos?
pital of Bengbu Medical College,China |
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Abstract: | Objective Objective To observe the effect of excretory/secretory products from Trichinella spiralis adult worms(AES)on
cecal ligation and puncture(CLP) ?induced sepsis in mice. Methods Methods Forty? ?eight BALB/c mice were randomly divided into 3
groups:a sham operation group(PBS + sham group,Group A),a CLP?induced sepsis group(PBS+ CLP group,Group B)and
an AES treatment group(AES+ CLP group,Group C) . The mice of each group were intraperitoneally injected with 25 μg of
AES or PBS only as a control in a total volume of 200 μl. Eight mice from each group were selected randomly for survival analy?
sis of 96 hours. The other 8 mice in each group were observed for pathological changes in the lung,liver and kidney tissues by
HE staining 12 h after CLP,and then determined for the detection of cytokines including TNF?α,IL?1β,IL?6,IL?10 and TGF?β in the sera by ELISA. Results Results The difference among the survival rates of mice in the 3 groups was statistically significant
(χ2
= 21.16,P < 0.05) . Compared to Group A(100%),the survival rate of mice in Group B(0)decreased significantly(P <
0.05),and also the pathological damage degrees in the lung,liver and kidney tissues of the mice in Group B increased signifi?
cantly after CLP. Compared with the mice in group B,the survival rate of those in Group C(70%)increased significantly(P <
0.05),and the pathological damage degrees in the lung,liver and kidney tissues of the mice in Group C decreased significantly
after the treatment with AES. The differences among the levels of pro?inflammatory cytokines TNF?α(F = 27.11,P < 0.05),IL?
1β(F = 18.75,P < 0.05)and IL?6(F = 100.93,P < 0.05)in the sera of the mice in the three groups were statistically signifi?
cant. Compared with the mice in Group A,the levels of the 3 cytokines of those in Group B increased significantly(all P <
0.05) . However,after the treatment with AES,the levels of the pro?inflammatory cytokines of those in Group C decreased signifi?
cantly(all P < 0.05) . The differences among the levels of immunoregulatory cytokines IL?10(F = 10.88,P < 0.05)and TGF?β
(F = 11.37,P < 0.05)in the sera of the mice in the three groups were also statistically significant. Compared with the mice in
Group B,the levels of IL?10 and TGF?β of those in Group C were higher after treatment with AES(both P < 0.05) . Conclu Conclu? ?
sion sion T. spiralis AES has a therapeutic potential for alleviating sepsis induced by CLP in mice. |
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Keywords: | Trichinella spiralis Cecal ligation and puncture(CLP)operation Excretory/secretory products Sepsis Pro? inflammatory cytokine Anti?inflammatory cytokine |
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