Tribbles同源蛋白1 rs2954029单核苷酸多态性与非酒精性脂肪性肝病及冠状动脉粥样硬化性心脏病的相关性分析

目的在青岛地区汉族人群中,探讨Tribbles同源蛋白1(TRIB1) rs2954029单核苷酸多态性与非酒精性脂肪性肝病(NAFLD)及冠状动脉粥样硬化性心脏病(CHD)的相关性,并探讨该基因多态性对血脂水平的影响。方法随机选取2017年1月-2017年11月收入青岛市市立医院的146例NAFLD患者为NAFLD组,155例CHD患者为CHD组,156例NAFLD合并CHD患者为合并组,175例健康对照人群为对照组。采集空腹静脉血进行生化检测和TRIB1 rs2954029基因型测定。应用χ^2检验分析样本是否符合Hardy-Weinberg平衡;计数资料组间比较采用χ^2检验;计量资料组间比较采用t检验或Wilcoxon秩和检验;比值比(OR)及其95%可信区间(95%CI)应用非条件logistic回归模型计算。结果 CHD组与对照组相比,合并组与NAFLD组相比,rs2954029位点基因型频率及等位基因频率分布差异均无统计学意义(P值均> 0.05)。CHD组与对照组中,rs2954029 AT+AA携带者与TT携带者相比,发病风险未增加(OR=1.496,95%CI:0.947~2.363,P=0.084);合并组与NAFLD组中,rs2954029 AT+AA携带者与TT携带者相比,发病风险未增加(OR=1.361,95%CI:0.832~2.227,P=0.219);校正性别、年龄和BMI后,差异仍无统计学意义(P值均> 0.05)。在全部人群、NAFLD组中,A等位基因携带者与非携带者间各生化指标差异均无统计学意义(P值均> 0.05);在CHD组和合并组中,A等位基因携带者的TG水平高于非携带者(Z值分别为-1.986、2.521,P值均<0.05);在对照组中,A等位基因携带者的TC水平高于非携带者(Z=-2.653,P <0.05)。结论青岛地区汉族人群中,TRIB1 rs2954029单核苷酸多态性未增加NAFLD患者发生CHD的发病风险。

Association of TRIB1 rs2954029 single nucleotide polymorphism with the risk of nonalcoholic fatty liver disease and coronary heart disease

Objective To investigate the association of TRIB1 rs2954029 single nucleotide polymorphism with nonalcoholic fatty liver disease(NAFLD) and coronary heart disease(CHD) and its influence on blood lipids in the Chinese Han population in Qingdao,China.Methods A total of 146 patients with NAFLD,155 patients with CHD,and 156 patients with NAFLD and CHD,who were admitted to Qingdao Municipal Hospital from January to November,2017,were enrolled as NAFLD group,CHD group,and NAFLD + CHD group,respectively,and 175 healthy individuals were enrolled as control group.Fasting venous blood samples were collected for biochemical analysis and TRIB1 rs2954029 genotyping.The chi-square test was used to analyze whether the distribution of genotypes was in accordance with the Hardy-Weinberg equilibrium.The chi-square test was used for comparison of categorical data between groups;the t-test and the Wilcoxon rank-sum test were used for comparison of continuous data between groups.The unconditioned logistic regression model was used to calculate odds ratio(OR) and its 95% confidence interval(CI).Results There were no significant differences in rs2954029 genotype/allele frequency and distribution between the CHD group and the control group,as well as between the NAFLD + CHD group and the NAFLD group(all P > 0.05).In the CHD group and the control group,rs2954029 AT + AA carriers did not have an increased risk of CHD compared with rs2954029 TT carriers(OR = 1.496,95% CI: 0.947-2.363,P = 0.084);in the NAFLD + CHD group and the NAFLD group,rs2954029 AT + AA carriers did not have an increased risk of CHD compared with rs2954029 TT carriers(OR = 1.361,95% CI: 0.832-2.227,P =0.219);there were still no significant differences after adjustment for sex,age,and body mass index.In the whole population and the NAFLD group,there were no significant differences in biochemical parameters between the individuals carrying A allele and those not carrying this allele(P > 0.05);in the CHD group and the NAFLD + CHD group,the individuals carrying A allele had a significantly higher level of triglyceride than those not carrying this allele(Z =-1.986,-2.521,all P < 0.05);in the control group,the individuals carrying A allele had a significantly higher level of total cholesterol than those not carrying this allele(Z =-2.653,P < 0.05).Conclusion In the Chinese Han population in Qingdao,TRIB1 rs2954029 single nucleotide polymorphism does not increase the risk of CHD in NAFLD patients.