miRNA21/PDCD4环路在卵巢癌组织中的表达

目的探讨miRNA21/PDCD4环路在卵巢肿瘤中的作用及其临床意义。方法采用qRT-PCR和Western blot方法检测miRNA21和PDCD4在108例卵巢癌患者、67例交界性病变、75例良性病变和35例正常组织中的表达情况。结果miRNA21在卵巢癌组织中的表达水平是正常卵巢组织的 12.3 倍(P＜0.01)；在交界性病变组织中的表达水平是正常卵巢组织的 7.8 倍 (P＜0.01)；在良性病变组织中的表达水平是正常卵巢组织的 3.6 倍 (P＜0.05)。PDCD4蛋白含量在卵巢癌组织中的表达明显低于交界性病变组织,交界性病变组织低于良性病变组织，良性病变组织低于正常卵巢组织，两两相比差异均具有统计学意义（P<0.05），正常卵巢组织与卵巢癌和交界性病变组织中PDCD4蛋白含量相比差异有统计学意义（P<0.01）。结论miRNA21/PDCD4环路异常在卵巢癌发生中具有重要的作用。

Expression of miRNA21/PDCD4 in Ovarian Cancer Tissues

Objective
To investigate the expression and clinical significance of miRNA21mRNA in ovarian cancer.
Methods
Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect miRNA21mRNA and PDCD4 protein levels in 108 cases of ovarian cancer patients,67 cases of borderline lesion,75 cases of benign lesion,and 35 cases of normal tissues.
Results
The expression levels of miRNA21mRNA in ovarian cancer,borderline lesion and benign lesion were significantly 11.3 times,6.8 times and 2.6 times respectively higher than those in normal tissues (P<0.01).The expression levels of miRNA21mRNA in borderline lesion were significantly 6.8 times higher than those in normal tissues (P<0.01,P＜0.01 and P＜0.05).The expression levels of PDCD4 in ovarian cancer borderline lesion,benign lesion and normal tissues were gradually and significantly decreased (P<0.05).
Conclusion
Up-regulated and down-regulated expressions of miRNA21mRNA and PDCD4 protein in ovarian cancer tissue might contribute to ovarian cancer carcinogenesis.