下调MLLT3/AF9基因对肝癌SMMC-7721细胞增殖与侵袭能力的影响

目的 本文旨在研究组蛋白甲基转移酶MLLT3/AF9在人肝癌组织中的表达，观察体外下调MLLT3/AF9的表达对肝癌SMMC-7721细胞增殖与侵袭能力的影响。方法 应用Real-time PCR技术检测100例肝癌组织、癌旁组织和肝癌细胞系SMMC-7721、HepG2、Hu7、BEL-7402中MLLT3/AF9基因的表达水平；应用RNA干扰下调MLLT3/AF9的表达，采用CCK-8、Transwell实验和划痕实验检测MLLT3/AF9下调组和对照组SMMC-7721细胞的增殖及侵袭能力。结果 Real-time PCR检测结果显示肝癌组织中的MLLT3/AF9基因的表达量显著高于癌旁组织； Real-time PCR和Western blot检测到LVMLLT3-RNAi组中MLLT3/AF9 mRNA和蛋白的表达水平都降低，CCK-8、Transwell和划痕实验结果显示LV-MLLT3-RNAi组细胞的增殖和侵袭能力降低。结论 MLLT3/AF9在肝癌组织中表达水平明显升高，体外下调MLLT3/AF9的表达可以有效抑制肝癌SMMC-7721细胞的增殖与侵袭。

Effect of Down-regulation of MLLT3/AF9 Expression on Proliferation and Invasion Abilities of Hepatoma SMMC-7721 Cells

Objective To investigate the expression of histone methyltransferase MLLT3/AF9 in human hepatocellular carcinoma (HCC) tissues and to observe the effect of down-regulation of MLLT3/AF9 expression in vitro on the proliferation and invasion of hepatocellular carcinoma SMMC-7721 cells. Methods Real-time PCR technique was used to detect the expression of MLLT3/AF9 gene in 100 cases of human hepatocellular carcinoma tissues and adjacent non-cancerous tissues and hepatocellular carcinoma cell lines SMMC-7721, HepG2, Hu7 and BEL-7402. RNA interference was used to reduce the expression of MLLT3/AF9. CCK-8, Transwell assay and scratch test were used to detect the proliferation and invasion abilities of SMMC-7721 cells in MLLT3/AF9 down-regulation group and control group. Results Real-time PCR results showed that the expression of MLLT3/AF9 in HCC tissues was significantly higher than that in paracancerous tissues. The mRNA and protein expression of MLLT3/AF9 in the experimental group were higher than those in the LVMLLT3- RNAi group. CCK-8, Transwell assay and scratch test results showed that the proliferation and invasion abilities of SMMC-7721 cells were decreased in LV-MLLT3-RNAi group. Conclusion The expression of MLLT3/AF9 in hepatocellular carcinoma tissues is significantly increased. The down-regulation of MLLT3/AF9 expression could effectively inhibit the proliferation and invasion of hepatoma SMMC-7721 cells.