黄芪多糖对气阴两虚Lewis肺癌荷瘤小鼠肿瘤生长、转移及细胞周期的影响

目的 观察黄芪多糖抑制气阴两虚Lewis肺癌荷瘤小鼠的生长、转移及对肺癌细胞周期的影响。方法 体外培养Lewis肺癌细胞，随机分为对照组和中药组，流式细胞术检测细胞周期；C57BL/6J小鼠90只，设空白组10只，余80只刨花烟熏并灌胃温热性的中药，移植Lewis肺癌实体肿瘤建立气阴两虚荷瘤小鼠模型并随机分为8组，比较各组小鼠抑瘤率及q值，计数外周血细胞及骨髓细胞，ELISA测定血清IL-2、IFN-γ、TNF-α、IL-10、HIF-1α、VEGF、MMP-2的含量。结果 黄芪多糖将Lewis 肺癌细胞阻滞于S期，随着药物浓度的增加，细胞凋亡逐渐增加；联合用药各组的抑瘤率高于黄芪多糖各组和顺铂组，q值均在0.85~1.15之间；与顺铂组比较，联合用药中、高剂量组小鼠外周血细胞及骨髓细胞的数量，IL-2、INF-γ、TNF-α均显著升高，IL-10、 HIF-1α、VEGF、MMP-2均显著降低（P<0.05, P<0.01）。结论 黄芪多糖在体外对Lewis肺癌细胞有抑制作用，体内与顺铂联合能抑制气阴两虚Lewis荷瘤小鼠肺癌细胞的生长、转移，提高外周血细胞和骨髓细胞的细胞数量，提高IL-2、INF-γ、TNF-α的含量，减少IL-10、HIF-1α、VEGF、MMP-2的含量。

Effects of Astragalus Polysaccharides on Growth,Metastasis and Cell Cycle of Lewis Lung Cancer in Tumor-bearing Mice with Qi and Yin Deficiency

Objective To observe the effects of Astragalus polysaccharides on the growth, metastasis and cell cycle of Lewis lung cancer in tumor-bearing mice with Qi and Yin deficiency. Methods Lewis lung cancer cells cultured in vitro were randomly divided into control group and Chinese medicine group. Cell cycle was detected by flow cytometry. Among 90 C57BL/6J mice, 10 mice were taken as blank group, and the other 80 mice were treated with Chinese medicine and gastric perfusion of warm smoked shavings. The mice bearing transplanted Lewis lung cancer solid tumor with Qi and Yin deficiency were randomly divided into eight groups. Mice tumor inhibition rate and q value were compared. Peripheral blood cells and bone marrow cells were counted and ELISA was applied to detect the content of serum IL-2, IFN-γ, TNF-α, IL-10, HIF- 1α, VEGF and MMP-2. Results Astragalus polysaccharide arrested Lewis lung cancer cells in S phase; with the increase of drug concentration, cell apoptosis increased gradually; the tumor inhibition rates in the drug combination groups were higher than those of Astragalus polysaccharide groups and cisplatin group, q value was 0.85-1.15; compared with cisplatin group, the number of peripheral blood cells and bone marrow cells, the content of IL-2, INF-γ and TNF-α in moderate- and high-dose drug combination groups were significantly increased, while the content of IL-10, HIF-1α, VEGF and MMP-2 were significantly decreased (P<0.05, P<0.01). Conclusion Astragalus polysaccharides in vitro has inhibitory effect on Lewis lung cancer cells; its combination with cisplatin in vivo could inhibit the growth and metastasis of Lewis lung cancer cells, increase the number of peripheral blood cells and bone marrow cells, the content of IL-2, INF-γ and TNF-α, and reduce the content of IL-10, HIF-1α, VEGF and MMP-2 in tumor-bearing mice with Qi and Yin deficiency.