| RORα高表达对人胃癌MGC803细胞增殖与迁移侵袭的影响 |
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| 引用本文： | 赵晓红,刘芳,向姝霖,夏红,曾希,苏波,凌晖,苏琦. RORα高表达对人胃癌MGC803细胞增殖与迁移侵袭的影响[J]. 肿瘤防治研究, 2016, 43(11): 926-932. DOI: 10.3971/j.issn.1000-8578.2016.11.002 |
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| 作者姓名： | 赵晓红 刘芳 向姝霖 夏红 曾希 苏波 凌晖 苏琦 |
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| 作者单位： | 1. 421001 衡阳，南华大学肿瘤研究所，湖南省胃癌研究中心，湖南省高校肿瘤细胞与分子病理学重点实验室；2. 570206 海口，海南省妇幼保健院妇产科；3. 418000 怀化，怀化市第一人民医院肿瘤科 |
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| 基金项目： | 国家自然科学基金（81374013, 81102854）；湖南省卫计委科研课题（B2015-182） |
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| 摘 要： | 目的 观察RORα高表达对MGC803细胞增殖与迁移侵袭的影响。方法 构建高表达RORα人胃癌MGC803细胞。Real-time PCR或RT-PCR和Western blot检测RORα、MMP-9与TIMP3 mRNA和蛋白表达。MTT、流式细胞术、迁移和侵袭实验检测RORα高表达对MGC803细胞增殖、细胞周期、迁移和侵袭能力的影响。结果 RORα高表达MGC803细胞RORα mRNA和蛋白表达显著上调。在48、72与96 h，RORα高表达细胞的增殖活性明显低于对照组和空载体组（P<0.05）。RORα高表达G2/M期细胞明显高于对照组和空载体组（P<0.05）。高表达组细胞的迁移距离较对照组与空载体组明显减少（P<0.05）。高表达组穿膜细胞数较对照组与空载体组明显减少（P<0.05）。RORα高表达可明显下调MMP-9和上调TIMP3mRNA与蛋白。结论 成功构建RORα高表达MGC803细胞，RORα高表达可抑制MGC803细胞增殖与迁移侵袭，将细胞阻滞于G2/M期，其机制可能与下调MMP-9和上调TIMP3有关。
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| 关 键 词： | 人胃癌MG C 8 0 3 细胞；ROR&alpha ；真核生物表达载体；增殖；迁移；侵袭 |
| 收稿时间： | 2016-01-11 |
Effect of RORα Overexpression on Proliferation,Migration and Invasion of Human Gastric MGC803 Cells |
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| ZHAO Xiaohong,LIU Fang,XIANG Shulin,XIA Hong,ZENG Xi,SU Bo,LING Hui,SU Qi. Effect of RORα Overexpression on Proliferation,Migration and Invasion of Human Gastric MGC803 Cells[J]. Cancer Research on Prevention and Treatment, 2016, 43(11): 926-932. DOI: 10.3971/j.issn.1000-8578.2016.11.002 |
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| Authors: | ZHAO Xiaohong LIU Fang XIANG Shulin XIA Hong ZENG Xi SU Bo LING Hui SU Qi |
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| Affiliation: | 1. Cancer Research Institute, Center for Gastric Cancer Research of Hu’nan Province, Key Laboratory of Cancer Cellular and Molecular Pathology of Hu’nan Provincial University, University of South China, Hengyang 421001; 2. Department of Gynaecology and Obstetrics, Hainan Maternal and Child Health Hospital, Haikou 570206, China; 3. Department of Oncology, Huaihua First People's Hospital, Huaihua 418000, China |
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| Abstract: | Objective To investigate the effect of RORα overexpression on human gastric cancer MGC803 cells proliferation, migration and invasion. Methods The expressions of RORα, MMP-9 and TIMP3 mRNA and protein were detected by Real-time PCR or RT-PCR and Western blot. The effect of RORα overexpression on the proliferation, cell cycle, migration and invasion of MGC803 cells were detected by MTT, flow cytometry, wound healing and Transwell assays. Results The expressions of RORα mRNA and protein were stably increased in the RORα/MGC803 cells. The proliferation activity in RORα/MGC803 cells were notably lower than in MGC803 cells and in vector/MGC803, respectively, at 48 h,72 h and 96 h(P<0.05). Percentage of G2/M in RORα/MGC803 cells markedly higher than that in MGC803 cells and vector/MGC803(P<0.05). The migration length in RORα/MGC803 cells was markedly lower than that in MGC803 and vector/MGC803 cells (P<0.05). The cells through the matrigel coated membrane in RORα/MGC803 significantly decreased comparing with MGC803 and vector/MGC803 cells(P<0.05). RORα overexpression was downregulation of MMP-9 and upregulation of TIMP3 mRNA and proteins(P<0.05). RORα overexpression could significantly downregulate MMP-9 and upregulate TIMP3 mRNA and proteins(P<0.05). Conclusion RORα/MGC803 cells with stably overexpressed RORα are successfully constructed. The overexpression of RORα may inhibit the proliferation, migration and invasion, as well as G2/M arrest in MGC803 cells, which may be correlated with the downregulation of MMP-9 and the upregulation of TIMP3. |
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| Keywords: | Human gastric MGC803 cells RORα Eukaryotic expression vector Proliferation Migration Invasion R735.2 |
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