HBV cccDNA的体外细胞模型和实验小鼠模型

HBV慢性感染仍然是世界性公共健康问题。现有抗病毒药物能够有效抑制HBV复制,却无法使之完全治愈。共价闭合环状DNA(cccDNA)是HBV复制的原始模版,具有显著的稳定性,是HBV持续感染的分子基础,也是实现感染治愈的关键靶点。由于较低的细胞内拷贝数,以及缺乏灵敏可靠的检测方法,限制了对HBV cccDNA生物合成、代谢和调控等方面的相关研究。建立合适的cccDNA研究模型有助于了解这些生物学过程。综述了近年来HBV cccDNA细胞培养模型和实验小鼠模型等方面的主要进展,以期为进一步研究HBV病毒学和抗病毒药物研发提供帮助。

In vitro cell model and mouse model of HBV cccDNA

Chronic hepatitis B virus(HBV) infection remains a major public health threat worldwide.Current antiviral drugs can effectively inhibit the replication of HBV,but they fail to achieve a complete cure.As the original template for HBV replication,covalently closed circular DNA(cccDNA) is intrinsically stable in vivo and is regarded as the molecular basis for persistent HBV infection and the key target for the cure of HBV infection.Studies on biosynthesis,metabolism,and regulation of HBV cccDNA have been limited by the low copy number of cccDNA within cells and the lack of sensitive and reliable detection methods.Establishment of appropriate research models of cccDNA helps to understand related biological processes.This article reviews the latest research advances in cell models and mouse models of HBV cccDNA,in order to facilitate future studies on HBV virology and development of antiviral drugs against HBV.