| 广佛手黄龙病的鉴定 |
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| 引用本文： | 袁蒙,潘利明,周良云,罗碧,王浩,杨全. 广佛手黄龙病的鉴定[J]. 中国实验方剂学杂志, 2020, 26(5): 117-122 |
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| 作者姓名： | 袁蒙 潘利明 周良云 罗碧 王浩 杨全 |
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| 作者单位： | 广东药科大学 中药学院, 国家中医药管理局 岭南药材生产与开发重点研究室, 国家中药材产业技术体系 广州综合试验站, 广东省南药规范化种植与综合开发工程技术研究中心, 广州 510006,广东药科大学 中药学院, 国家中医药管理局 岭南药材生产与开发重点研究室, 国家中药材产业技术体系 广州综合试验站, 广东省南药规范化种植与综合开发工程技术研究中心, 广州 510006,广东药科大学 中药学院, 国家中医药管理局 岭南药材生产与开发重点研究室, 国家中药材产业技术体系 广州综合试验站, 广东省南药规范化种植与综合开发工程技术研究中心, 广州 510006,广东药科大学 中药学院, 国家中医药管理局 岭南药材生产与开发重点研究室, 国家中药材产业技术体系 广州综合试验站, 广东省南药规范化种植与综合开发工程技术研究中心, 广州 510006,广东药科大学 中药学院, 国家中医药管理局 岭南药材生产与开发重点研究室, 国家中药材产业技术体系 广州综合试验站, 广东省南药规范化种植与综合开发工程技术研究中心, 广州 510006,广东药科大学 中药学院, 国家中医药管理局 岭南药材生产与开发重点研究室, 国家中药材产业技术体系 广州综合试验站, 广东省南药规范化种植与综合开发工程技术研究中心, 广州 510006 |
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| 基金项目： | 国家重点研发计划项目（2017YFC1700704）；广东省中医药局中药资源普查专项（20163008）；2017年广东省岭南中药材保护资金专项（粤财社[2017]60号） |
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| 摘 要： | 目的:该文从多方面进行分析、比较,及时、准确地鉴别广佛手黄龙病,以便于及时确定病害、防控病情。方法:通过性状分析,逆转录-聚合酶链式反应(RT-PCR),酶切反应及实时荧光定量PCR(Real-time PCR)检测多种手段相结合,对广佛手黄龙病进行鉴定。结果:从性状上来看,感病广佛手有典型的叶片斑驳型黄化、果相对较小,甚至畸形的特征,但并没有发现"红鼻子果"现象,这些均可以作为田间初步判别广佛手黄龙病的依据; RT-PCR检测及酶切反应结果表明,引物为OI1/OI2c时,广佛手病株有特异性条带1 160 bp,且能被Xba I酶切成520 bp和640 bp,这与其他被黄龙病侵染的柑橘属植物的检测结果一致; Real-time PCR检测结果显示,被黄龙病侵染的广佛手叶片有扩增曲线和熔解曲线,熔链温度为82℃,且Ct值在24. 6～28. 2,而广佛手正常株没有扩增现象出现。结论:性状分析可以在田间初步判别黄龙病,但是具有一定的主观性,RTPCR及Real-time PCR检测能进一步确定广佛手黄龙病,且q PCR检测更加灵敏,还可以定量。通过性状分析以及分子鉴定的结合,能够更加及时、准确地确定广佛手黄龙病。
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| 关 键 词： | 广佛手 黄龙病 叶片 性状分析 田间判别 |
| 收稿时间： | 2019-02-22 |
Identification of Citrus medica var. sarcodactylis Infected with Citrus Huanglongbing |
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| YUAN Meng,PAN Li-ming,ZHOU Liang-yun,LUO Bi,WANG Hao and YANG Quan. Identification of Citrus medica var. sarcodactylis Infected with Citrus Huanglongbing[J]. China Journal of Experimental Traditional Medical Formulae, 2020, 26(5): 117-122 |
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| Authors: | YUAN Meng PAN Li-ming ZHOU Liang-yun LUO Bi WANG Hao YANG Quan |
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| Affiliation: | Guangdong Provincial Research Center on Good Agricultural Practice&Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, Guangzhou Comprehensive Experimental Station of China Chinese Materia Medica Industry Technology System, Key Laboratory for Production&Development of Cantonese Medicinal Materials Under State Administration of Traditional Chinese Medicine(TCM), School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China,Guangdong Provincial Research Center on Good Agricultural Practice&Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, Guangzhou Comprehensive Experimental Station of China Chinese Materia Medica Industry Technology System, Key Laboratory for Production&Development of Cantonese Medicinal Materials Under State Administration of Traditional Chinese Medicine(TCM), School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China,Guangdong Provincial Research Center on Good Agricultural Practice&Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, Guangzhou Comprehensive Experimental Station of China Chinese Materia Medica Industry Technology System, Key Laboratory for Production&Development of Cantonese Medicinal Materials Under State Administration of Traditional Chinese Medicine(TCM), School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China,Guangdong Provincial Research Center on Good Agricultural Practice&Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, Guangzhou Comprehensive Experimental Station of China Chinese Materia Medica Industry Technology System, Key Laboratory for Production&Development of Cantonese Medicinal Materials Under State Administration of Traditional Chinese Medicine(TCM), School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China,Guangdong Provincial Research Center on Good Agricultural Practice&Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, Guangzhou Comprehensive Experimental Station of China Chinese Materia Medica Industry Technology System, Key Laboratory for Production&Development of Cantonese Medicinal Materials Under State Administration of Traditional Chinese Medicine(TCM), School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China and Guangdong Provincial Research Center on Good Agricultural Practice&Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, Guangzhou Comprehensive Experimental Station of China Chinese Materia Medica Industry Technology System, Key Laboratory for Production&Development of Cantonese Medicinal Materials Under State Administration of Traditional Chinese Medicine(TCM), School of TCM, Guangdong Pharmaceutical University, Guangzhou 510006, China |
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| Abstract: | |
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| Keywords: | Citrus medica var. sarcodactylis Huanglongbing(HLB) leaves character analysis distinguish in field |
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