协同刺激分子CD86在母-胎免疫调节中的作用

目的　探讨协同刺激分子CD86在母 胎免疫调节中的作用。方法　建立自然流产模型(CBA×DBA/ 2 )及正常妊娠模型 (CBA×BALB/c)。两种模型分别再分为 3个组 :(1)以大鼠的IgG为对照的对照组 ;(2 )于妊娠第 4、6、8、10天 ,分别给CBA孕鼠腹腔注射大鼠抗小鼠CD86单克隆抗体的多次干预组 ;(3)仅于妊娠第 4天 ,给CBA孕鼠单次腹腔注射抗CD86单克隆抗体的单次干预组。每次腹腔注射的抗体剂量均为 10 0 μg。于妊娠第 14天计算各组胚胎吸收率。 结果　 (1)自然流产模型与正常妊娠模型的对照组胚胎吸收率分别为 2 7 78%和 8 42 %。 (2 )于妊娠第 4、6、8、10天腹腔注射抗CD86单克隆抗体后 ,自然流产模型的多次干预组胚胎吸收率下降至 9 6 8% (P <0 0 5 ) ;而正常妊娠模型的多次干预组胚胎吸收率上升至 13 5 4% (P >0 0 5 )。 (3)妊娠第 4天单次腹腔注射抗CD86单克隆抗体 ,自然流产模型单次干预组的胚胎吸收率下降至 7 14% (P <0 0 0 1) ;而正常妊娠模型单次干预组的胚胎吸收率上升至 11 39% (P >0 0 5 )。结论　妊娠早期 ,尤其在胚胎对母体的致敏阶段 (着床期 ) ,阻断母 胎界面的CD86协同刺激信号 ,将诱导母体对胚胎的免疫耐受 ,从而减少胚胎吸收 ,提高妊娠成功率

Role of costimulating molecule CD86 in maternal-fetal immune regulation

OBJECTIVE: To evaluate the role of costimulating molecule CD86 in maternal-fetal immune regulation. METHODS: In abortion-prone (CBA x DBA/2) matings and non-abortion-prone (CBA x BALB/c) matings, the pregnant CBA females were injected intraperitoneally by purified rat anti-murine CD86 monoclonal antibody (mAb) with dosage of 100 micrograms at day 4, 6, 8 and 10 of gestation respectively, or injected only once at day 4 of gestation, or injected with purified rat IgG as control. The resorbing and non-resorbing embryos were enumerated at day 14 of gestation. RESULTS: The embryo resorbing rate in CBA x DBA/2 matings was 27.78%, and that in CBA x BALB/c matings was 8.42%. After injection of anti-CD86 mAb at day 4, 6, 8, 10 of gestation, the embryo resorbing rate reduced to 9.68% (P < 0.005) in CBA x DBA/2 matings, while that was 13.54% in CBA x BALB/c matings (P > 0.05). After injection of anti-CD86 mAb only once at day 4 of gestation, the embryo resorbing rate reduced apparently to 7.14% (P < 0.001) in CBA x DBA/2 matings, while that was 11.39% (P > 0.05) in CBA x BALB/c matings. CONCLUSIONS: Blocking the costimulating signal by anti-CD86 mAb at maternal-fetal interface, especially at the prime stage of maternal-fetal recognition (periods of implantation), may result in maternal-fetal tolerance, and hopefully the embryo resorption turns to normal pregnancy.