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| 总序香茶菜的组织培养与快繁研究 | |
| 引用本文： | 丁兰,吕军旺,汪汉卿. 总序香茶菜的组织培养与快繁研究[J]. 中草药, 2005, 36(1): 115-117 |
| 作者姓名： | 丁兰 吕军旺 汪汉卿 |
| 作者单位： | 1. 中国科学院兰州化学物理研究所OSSO国家重点实验室,甘肃,兰州,730000;西北师范大学生命科学学院,甘肃,兰州,730070 2. 西北师范大学生命科学学院,甘肃,兰州,730070 3. 中国科学院兰州化学物理研究所OSSO国家重点实验室,甘肃,兰州,730000 |
| 基金项目： | 黑龙江省教委重大资助项目(9551Z002) |
| 摘 要： | 目的对总序香茶菜进行组织培养和离体快速繁殖。方法以总序香茶菜带芽茎段为外植体,在附加有不同种类和不同浓度激素的MS培养基上进行培养。结果最佳芽诱导培养基:MS BA0.05～0.5mg/L NAA0.01～0.05mg/L;最佳芽增殖培养基:MS BA0.5mg/L NAA0.05mg/L(或IAA0.01mg/L);最佳生根培养基:MS NAA0.1mg/L 活性炭0.05%;最佳愈伤组织诱导与生长培养基为:MS 2,4-D1.0～2.0mg/L BA0.1～0.2mg/L。结论成功建立了快速无性繁殖系。 |
| 关 键 词： | 总序香茶菜 组织培养 快速无性繁殖系 愈伤组织 |
| 文章编号： | 0253-2670(2005)01-0115-03 |
| 收稿时间： | 2004-03-07 |
Tissue culture and rapid propagation of Rabdosia racemosa |
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| DING Lan,LU Jun-wang and WANG Han-qing. Tissue culture and rapid propagation of Rabdosia racemosa[J]. Chinese Traditional and Herbal Drugs, 2005, 36(1): 115-117 | |
| Authors: | DING Lan LU Jun-wang WANG Han-qing |
| Affiliation: | DING Lan,L(U) Jun-wang,WANG Han-qing |
| Abstract: | Objective To analyze the sibling relationship and genetic diversity of four certified Radix Gentianae (RG) species by RAPD and ISSR methods. It provided molecular biological proof for species identification and breeding of certified RG. Methods PCR reaction systems of RAPD and ISSR were optimized, and the agarose gel electrophoresis results were analyzed into statistic data. Results Ten RAPD primers and four ISSR primers were selected respectively from 100 RAPD primers and 32 ISSR primers. The statistic data were analyzed to construct cluster analysis dendrogram by genetic distance UPGMA method. Conclusion The PCR reaction systems of this experiment gets ideal RAPD and ISSR results that suit to the analysis of certified RG species in genetic diversity and sibling relationship. |
| Keywords: | Rabdosia racemosa (Hemsl.) tissue culture rapid propagation callus |
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