旋毛虫感染小鼠IgG、IL-2和T淋巴细胞亚群动态变化的观察

目的　了解小鼠感染旋毛虫后 Ig G抗体水平、IL - 2分泌量和 T淋巴细胞亚群的动态变化。方法　分别于旋毛虫感染后第 7、14、2 1、2 8和 35天 ,采用 EL ISA方法检测血清特异性 Ig G抗体水平和 IL- 2含量 ,采用流式细胞仪检测 CD4+、CD8+T细胞百分率。结果　 Ig G抗体水平在感染后逐渐上升 ,感杂后 35天达最高值 ;T淋巴细胞的变化表现为 CD4+T细胞减少、CD8+T细胞增多 ,CD4+/ CD8+细胞比值下降 ,以感染后第 14天最为显著 ,直到感染后第 35天仍未见恢复。IL - 2分泌量以感染后第 7天达高峰 ,然后迅速下降 ,到感染后第 35天低于正常组。讨论　旋毛虫感染的急性期 ,小鼠出现免疫抑制现象。抗旋毛虫感染的保护性免疫是由细胞免疫与体液免疫协同完成的。

OBSERVATION OF THE KINETIC CHANGE ON IgG,T-CELL SUBSETS,IL-2 LEVEL OF MICE INFECTED TRICHINELLA SPIRALIS

Aim\ To explore the kinetic change of the IgG,T-cell subsets and IL-2 level from the mice infected with T.Spiralis. Methods The level of specific IgG A band IL-2 was determined by ELISA,the percentage of CD4 and CD8 T-cells were examined by flow cytometry on 7,14,21,28,35 days after mice infected with T.spiralis respectively.Result\ After infected with T.spiralis,the level of IgG in mice rised gradually,and reached its peak on the 35 th day.The change of T-cell subsets showed that CD4 T cells decreased,which CD8 Tcells increased.The ration of CD4 /CD8 cells decreased,and which was the obvious on the 14 thday.It did not recover to normal level even on the 35 thday.The IL-2 of level reached the peak on the 7 thday after infected,then IL-2 level decreased quickly and lower than that of normal mice on 35 th day after infected.Conclusion When the acute phase of T.spiralis infection,the immune function of host was inhibited.The protective immunity of against T.spiralis infection was cellular immunity mainly,in cooperation with humoral immunity.