小鼠睾丸支持细胞的体外分离、培养与鉴定

目的：用改良方法对小鼠睾丸支持细胞进行体外快速分离与培养，以期建立一种简单快捷纯度高的支持细胞原代培养方法。方法：用0．25％胰蛋白酶和0．1％胶原酶，对睾丸组织进行次第消化，用福尔根染色法和免疫细胞化学方法鉴定睾丸支持细胞。结果：细胞培养72h存活率超过90％，纯度在85％以上。福尔根染色和免疫细胞化学染色结果示，分离细胞有卫星核小体存在，且细胞中FasL阳性表达，表明分离培养的细胞确为睾丸支持细胞。结论：睾丸组织经曲细精管分离后进行胶原酶和胰蛋白酶次第消化，可快速高效分离睾丸支持细胞。

Separation, Culture and Identification of Mice Sertoli Cells in vitro

Objective:To use the modified method to separate and culture sertoli cells in order to set up a simple and effective method for separating sertoli cells.Method:The tissue was treated with the 0.25% collagenase and 0.1% trypsin sequentially.Sertoli cells were identified according to Feulgen staining and immunocytochemistry.Results:The cell viability was over 90% and the purity was over 85% after 72 hours.The satellite karyosomes were stained inviolet.The expression of FasL was examined by SABC staining.Concl...