出生后发育不同阶段大鼠眼内nestin阳性表达的变化

目的 观察SD大鼠出生后的不同发育阶段眼内nestin阳性表达的部位、数量变化.方法 选取出生1、7、14、21、30d SD大鼠,通过免疫细胞化学方法检测nestin在大鼠眼内的阳性表达.结果 在出生后SD大鼠眼内视网膜及眼外肌均有nestin阳性表达,且随发育时间变化,在视网膜由各层分布到仅在神经纤维层偶有表达,而在眼外肌表达量逐渐减少.结论 出生后大鼠随年龄增长,视网膜及眼肌nestin阳性表达量逐渐减少.

Variation of nestin expression in SD rat eyes in different stages of postnatal development

Objective To observe the position and quantity of nestin expression in SD rat eyes in different stages of postnatal development. Methods Immunocytochemical method was used to identify nestin expression in the eyes of SD rats of 1 to 30 days old. Results Nestin expression was detected in the retina and extraocular muscles of SD rats. The expression varied with the time of postnatal development, distributing in the entire retina layers in earlier stages and confined in the nerve fiber layer in later stages. The quantities of nestin expression in the extraocular muscles decreased gradually with growth. Conclusion Nestin expression in the retinas and extraocular muscles of SD rats decreases during the postnatal development.