血管内皮生长因子基因转染对肝细胞移植的影响

目的探讨VEGF基因转染对移植后血管组织形成的作用及对移植细胞增殖的影响.方法SD大鼠pcDNA3VEGF121转染肝细胞移植10d后取样,行微血管密度(MVD)计数及增殖细胞核抗原(PCNA)染色.结果在体外VEGF转染肝细胞能产生足够的转基因蛋白诱导内皮细胞的增殖.而体内可形成大量移植细胞克隆及再生肝组织.各组MVD计数差异并无显著性,P>005;在VEGF基因转染组PCNA指数较pcDNA3对照组与非转染组显著升高,为13.13±275、475±1.58和4.63±1.41,P<0.01.提示在体内移植血管组织形成存在多重因素的影响.而VEGF基因表达可促进移植细胞增殖和再生肝组织形成.结论VEGF间接体内基因转染是诱导移植肝再生一有效方法.

Effect of vascular endothelial growth factor gene transfection on hepatocyte transplantation

OBJECTIVE: To investigate the effect of vascular endothelial growth factor(VEGF) gene transfection on tissue vascularization in transplanted site and the proliferation of transplanted hepatocytes. METHODS: Samples were gained at ten days after the pcDNA3 VEGF121 gene transfected hepatocyte was transplanted into the spleen of SD rats. The changes of microvascular density(MVD) counts in the transplanted site and proliferating cell nuclear antigen(PCNA) index of the transplanted hepatocytes were examined. RESULTS: In vitro protein expression of VEGF gene was enough to induce proliferation of human umbilical venous endothelial cells. In vivo VEGF gene transfected hepatocytes could form mass colonization of transplanted hepatocytes and reconstituted liver tissue. Difference of MVD counts in the transplanted site on all groups were not significant. P>0.05; PCNA index of pcDN-A3 VEGF121 gene transfection group increased significantly compared with pcDNA3 control group and no transfection group, P<0.01. PCNA index were 13.13 +/- 2.75, 4.75 +/- 1.58, and 4.63 +/- 1.41, suggesting that in vivo many factors affect the tissue vascularization, and expression of VEGF gene can promote not only proliferation of transplanted hepatocyte but formation of reconstituted liver tissue. CONCLUSIONS: VEGF ex vivo gene transfection is an effective way to induce reconstitution of transplanted liver tissue.