| 同源重组修复基因RAD51可提高PTEN缺失细胞基因组稳定性 |
| |
| 引用本文： | 陈忠民,徐勤枝,胡迎春,霍艳英,周平坤,吴德昌.同源重组修复基因RAD51可提高PTEN缺失细胞基因组稳定性[J].癌变．畸变．突变,2011,23(6):406-409. |
| |
| 作者姓名： | 陈忠民 徐勤枝 胡迎春 霍艳英 周平坤 吴德昌 |
| |
| 作者单位： | 1. 军事医学科学院放射与辐射医学研究所,北京100850;中国人民解放军第二炮兵防疫中心,北京100071
2. 军事医学科学院放射与辐射医学研究所,北京,100850 |
| |
| 基金项目： | 国家重大科学研究计划(2007CB914603);国家自然科学基金项目(30872136) |
| |
| 摘 要： | 目的：研究同源重组修复基因RAD51对PTEN缺失小鼠胚胎成纤维细胞基因组稳定性的影响。方法：应用免疫荧光和中性单细胞电泳技术观察PTEN缺失后自发性和辐射诱导DNA双链断裂情况;并构建PTEN缺失细胞稳定转染RAD51的细胞系,γ射线照射后检测细胞存活率。结果：PTEN缺失导致细胞自发性和辐射诱导DNA双链断裂增多;转染RAD51后细胞存活率增高,辐射诱导的DNA双链断裂减少。结论：同源重组修复基因RAD51可以提高PTEN缺失细胞基因组稳定性。
|
| 关 键 词： | PTEN RAD51 基因组稳定性 |
Homologous recombination repair gene RAD51 could increase the genomic stability of PTEN deleted cells |
| |
| CHEN Zhong-min,XU Qin-zhi,HU Ying-chun,HUO Yan-ying,ZHOU Ping-kun,WU De-chang.Homologous recombination repair gene RAD51 could increase the genomic stability of PTEN deleted cells[J].Carcinogenesis,Teratogenesis and Mutagenesis,2011,23(6):406-409. |
| |
| Authors: | CHEN Zhong-min XU Qin-zhi HU Ying-chun HUO Yan-ying ZHOU Ping-kun WU De-chang |
| |
| Institution: | 1 (1.Institute of Radiation Medicine,Academy of Military Medical Science,Beijing 100850;2.Center for Disease Control And Prevention,Second Artillery Force of PLA,Beijing 100071,China) |
| |
| Abstract: | OBJECTIVE:To explore the effect of genomic stability of PTEN-deficient mice embryonic fibroblasts after transfection with RAD51,an important homologous recombination repair gene.METHODS: Immunofluorescence assay was used to observe the number of spontaneousγ-H2AX foci.Neutral single cell gel electrophoresis was applied to detect spontaneous and radiation-induced DNA double-strand breaks in both PTEN-deficient and PTEN wild-type cells.RESULTS:PTEN deletion led to an increase of spontaneousγ-H2AX foci and the length of tail moment,indicating DNA double-strand breaks.Transfection with RAD51 increased cell survival and decreased radiation-induced DNA double-strand breaks of PTEN-deficient cells.CONCLUSION:Homologous recombination gene RAD51 could increase genomic stability of PTEN-deficient cells. |
| |
| Keywords: | PTEN RAD51 genomic stability |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
