独一味胶囊对大鼠动脉管壁增殖细胞核抗原表达的影响

目的：观察藏药独一味胶囊对经皮冠状动脉成形术（percutaneous transluminal coronary angioplasty,PTCA）大鼠动脉管壁增殖细胞核抗原（proliferating cell nuclearantigen,PCNA）表达的影响。方法：Wistar大鼠40只随机分为PTCA模型组（同时设右侧相同部位的颈总动脉为正常对照）、独一味胶囊0.50、1.25、2.50g/kg组、阳性对照组（丹参片1.0g/kg）,共5组,每组8只。各组动物灌胃给予相应药物第5天后行PTCA术,建立颈动脉球囊损伤模型;PTCA术后继续给予相应药物28 d后取损伤节段颈总动脉,常规病理切片后行免疫组织化学检测,测定PCNA的表达。结果：正常对照管壁PCNA表达极少或无表达。模型组动脉内膜PCNA表达的阳性率为33.71%;独一味胶囊0.50、1.25、2.50g/kg组动脉内膜PCNA阳性率依次为28.00%、27.83%、27.33%,与模型组比较均显著降低（P〈0.05或P〈0.01）。结论：独一味胶囊可显著抑制PCNA的表达,可能是预防大鼠颈动脉损伤后再狭窄的机制之一。

Effect of Duyiwei capsule on proliferating cell nuclear antigen expression in rats

OBJECTIVE:To investigate the effect of Duyiwei(lamiophlomis rotate) capsule on the expression of proliferating cell nuclear antigen(PCNA) in Wistar rats.METHODS:40 Wistar rats,half male half female,were randomly divided into 5 groups:model group,PTCA group,Duyiwei(Lamiophlomis rotate) capsule 0.50,1.25,2.50 g/kg,positive control group(salvia miltrorrhiza tablet 1.0 g/kg).Oral administration of drugs was performed in each group,continuously 5 days.Then all rats were treated by percutaneous transluminal coronary angioplasty(PTCA) for the establishment of carotid balloon injury model.Then drug treatment continued after PTCA for 28 days.Carotid artery segment underwent routine biopsy immunohistochemistry and PCNA expression determination.RESULTS:In the normal artery,these was low level or no expression of PCNA.In the arterial intima of the model groups,33.71%was PCNA-positivewith each dose of Duyiwei capsule group showing significant differences(P<0.05).CONCLUSION:Duyiwei capsule significantly inhibited the expression of PCNA,which may be one of its mechanisms in preventing restenosis after carotid artery injury in rats.