白血病hZIP1,ZnT1基因表达特点及上清锌含量测定的实验研究

目的:研究锌转运体在白血病各阶段的变化规律及与白血病疾病发展过程的相关性,探讨锌浓度及其转运体对造血调控作用的影响.方法:将实验对象分为急性髓系自血病组、慢性粒细胞白血病组、正常组、细胞株组;采用半定量RT-PCR的方法测定人类锌铁调控蛋白(human ZRT/IRT-like protein,hZIP1)及阳离子转运促进因子1(cation diffusion facilitator,CDF,又称ZnT1)mRNA的表达:应用火焰原子吸收法测定骨髓上清巾的锌含量.结果:hZIP1基因在骨髓单个核细胞上阳性表达,但在急、慢性白血病的各个阶段的表达均无显著性差异(P>0.05);ZnT1基因在骨髓单个核细胞上阳性表达,初治、复发组白血病患者ZnT1表达量明显高于正常对照和缓解组(P<0.05);在K562、HL60细胞株中的实验结果与临床标本的实验结果一致;急性白血病初治组和复发组骨髓上清锌含量明显高于缓解组和正常组(P<0.05).结论:hZIP1基因与细胞内外的锌水平调节有关,但与白血病时细胞内外锌含昔变化可能无明显关系:ZnT1与白血病骨髓单个核细胞中锌水平调节密切相关,是调节细胞内外锌含量的重要基因;ZnT1表达水平降低、骨髓上清锌含量降低提示病情好转.

The experimental research on mRNA expressions of hZIP1 and ZnT1, and Zinc level of bone marrow in leukaemia

Objective:To investigate expressions of human ZRT/IRT-like protein(hZIP1) and cation diffusion facilitator(CDF,ZnT1) mRNAs, study the relationship between Zinc,Zinc transporters and leukemia, and understand its application at precaution,diagnosis and therapy of leukemia. Methods:the experiment objects were divided into the acute myelocytic group, chronic myelocytic leukemia, control group and cell line group. Expressions of hZIP1 and ZnT1 mRNAs were analyzed by RT-PCR. Zinc level in supernatant of bone marrow were measured by flame atomic absorption spectrometry(FAAS) expression. Results:Expression of hZIP1 mRNA of the mononuclearcell in bone marrow was detected. There was no significant difference among incipient group,relapsed group,complete remission group and control group(P > 0.05). Expression of ZnT1 mRNA of the mononuclearcell in bone marrow was detected,and its expression in incipient group and relapsed group were remarkably higher than those in complete-remission group and control group(P < 0.05). The results in cell strains, K562 and HL60, accorded with those in clinical collections. The Zinc levels in supernatant of bone marrow in incipient group and relapsed group were remarkably higher than those in control group and complete remission group(P < 0.05). Conclusion:The hZIP1 plays role in regulating Zinc balance of cells inside and outside,but it has no significant relevance with Zinc regulation when leukeamia occurs. ZnT1 is a significant gene in regulating Zinc level, and has significant relevance with Zinc regulation of the mononuclearcell in bone marrow when leukeamia occurs. The expression of ZnT1 in the bone marrow and the Zinc level in supernatant of bone marrow decreased after therapy suggesting the improvement condition of patients.