骨痹消对大鼠佐剂性关节炎血清中TNF-α、IL-1β、IL-6水平的影响

目的：观察骨痹消对关节损伤的防治作用及机制，为AS的治疗提供实验依据。方法：取Wistar雄性大鼠40只，随机分为4组，每组10只。分别为模型组、中药组、罗非西布组、对照组。用佛氏佐剂注射于模型组、中药组、罗非西布组大鼠左后足掌皮下，造佐剂性关节炎模型；对照组注射等量生理盐水。中药组和罗非西布组分别灌胃给中药水煎剂2ml／200g、罗非昔布2ml／200g（0．2mg），模型组和对照组灌胃给自来水2ml／200g，每天灌胃1次。结果：在使用中药骨痹消方后，大鼠血清TNF-α、IL-1β、IL-6水平均显著下降。结论：中药骨痹消对大鼠佐剂性关节炎有一定的治疗作用，可减轻炎症反应和关节肿胀，改善关节功能，其机制可能与下调TNF-α、IL-1β、IL-6，抑制炎症反应有关。

Effect of Gu Bi Xiao on TNF-а, IL-1β, IL-6 in Rats with AIA

Objective：To explore preventive and treatment effects of Gu Bi Xiao on joint injuries and its mechanism, providing lab data for Ankylosing Spondylitis treatment. Methods：Forty Wister male rats were randomly divided into 4 groups （n = 10） ： the model group, Chinese medicine group, Rofecoxib group, the three of which established AIA model by injecting Freud＇s Adjuvant into the plantar surface of left hind paw, and control group injected with same volume of Sodium Chloride. Chinese medicine decoction （2mL/200g） and Rofecoxib （2mL/200g）, or tap water （2mL/200g） were administered respectively to four groups by gavages daily. Results ：TNF-a, IL-1β, IL-6 in Rats with AIA decreased markedly after dministration of Gu Bi Xiao. Conelusion：Gu Bi Xiao is effective in the treatment of AIA by relieving inflammation and joint swelling and improving joint functions, which may have something to do with down-regulation of TNF-a, IL-1β, IL-6.