芒果甙对肝癌大鼠肝组织β-catenin和p120ctn表达的影响

目的　探讨芒果甙抗肿瘤作用及其机理 ,检测肝癌组织中 β -catenin和p12 0ctn的表达以及芒果甙对上述分子表达的影响。方法　采用Walker2 5 6肝癌细胞和Wistar大鼠制作肝癌模型 ,用免疫组化法观察肝组织中 β -catenin和p12 0ctn的表达情况。 结果　A组肝组织中上述分子表现为胞膜表达而胞浆无表达。B组中主要表现为胞膜胞浆均表达或胞膜表达减弱 ,一些细胞胞膜缺乏表达。C组中主要表现为胞膜表达明显 ,少数细胞胞浆也有表达。D组中主要表现为胞膜胞浆均明显表达 ,但以胞膜为主 ,少数细胞缺乏表达。E组中主要表现为胞膜胞浆均出现表达 ,且以胞浆表达为主 ,少数细胞出现胞膜表达减弱且不连续。结论　芒果甙对这些细胞信号转导分子的正常表达有一定的稳定作用。提示芒果甙是一个具有针对介导的细胞粘附和信号转导通路的潜在的抗癌物质。

Effect of Mangiferin on the Expression of β-cateninand p120ctn in Hepatic Tissues of Rats with Liver Cancer

Objective To explore the antitumor effects and mechanism of Mangiferin, detect the expression of β-catenin and p120ctn in tissues of liver cancer and how Mangiferin effects on the expressions of these molecules. Method Used cells of liver cancer of Walker 256 and Wistar rats for liver cancer model and observed expressions of β-catenin and p120ctn in hepatic tissues by immunohistochemical techniques. Result Group A: The expressions of the above cells were of cytomembrane while no expressions for kytoplasm; Group B: Expressions of both cytomembrane and kytoplasm or decrease of that of kytoplasm were detected while some cytomembranes lacked expression; Group C: Obvious expressions of cytomembrane and certain kytoplasms were detected; Group D: Obvious expressions of both cytomembrane and kytoplasm with cytomembrane dominating, some cells showed no expressions; Group E: Expressions of both cytomembrane and kytoplasm appeared with kytoplasm dominating, decrease of expressions and discontinuity occurred. Conclusion Mangiferin is of definite stabilization in the normal expressions of cell signal transduction prompting that it possesses potential antitumor substances in the cell adhesion and channels of signal transduction of mediate.