亚砷酸联合顺铂对人鼻咽癌裸鼠移植瘤及增殖细胞核抗原和Ki-67的影响

目的　研究亚砷酸（As2O3）对人鼻咽癌CNE-2Z裸鼠移植瘤与增殖细胞核抗原（proliferating cell nuclearantigen，PCNA）、Ki-67的作用，探讨该药物的抗肿瘤机制。方法　建立人鼻咽癌裸鼠移植瘤模型，随机分为对照组（NaCl）组、As2O3组、顺铂（Cisplatin，DDP）组和As2O3+DDP组，观察裸鼠移植瘤生长情况；采用HE染色，于光镜下观察各组移植瘤组织细胞病理形态学变化，同时观察心、肝、肺、肾组织有无病理学改变及瘤细胞转移。进行血常规检测，采用免疫组织化学方法检测PCNA、Ki-67的表达。结果　As2O3、DDP及两药联用均明显抑制人鼻咽癌裸鼠移植瘤生长，裸鼠的心、肝、肺和肾未见病理学改变和瘤转移，联合用药未增加造血系统的毒性，并能下调PCNA和Ki-67的表达。结论　亚砷酸可抑制人鼻咽癌CNE-2Z裸鼠移植瘤的生长，此作用可能与下调PCNA、Ki-67的表达相关。

Effects of arsenic trioxide combined with cisplatin on expression of PCNA and Ki-67 in human nasopharyngeal carcinoma xenograft in nude mice

[ABSTRACT]OBJECTIVETo study the effects of Arsenic Trioxide on the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, and explore the possible mechanisms of the antitumor effect. METHODSThe models of human poorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into 4 groups (control group, As2O3 group, DDP group and As2O3+DDP group). The antitumor effect of every group was studied. Pathological changes of tumor tissues dyed by HE staining were observed with light microscope. The pathological changes of the heart, liver, lung and kidney from nude mice were also observed by HE staining. Blood routine test was examined. Expression of PCNA and Ki-67 were detected by immunohistochemistry method.RESULTSThe growth of tumor in As2O3, DDP and As2O3+DDP were obviously inhibited. No pathological changes and metastases were found in heart, liver, lung and kidney of the nude mice. No obvious toxicity of the treatment to the hematopoietic systems in the nude mice was observed. The expression of PCNA and Ki-67 were down-regulated.CONCLUSIONAs2O3 can inhibit the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, the mechanism of which might be related to the down-regulation of PCNA and Ki-67.