肝细胞癌肿瘤抑制基因P53改变及其与乙型肝炎病毒关系的研究

肝细胞癌肿瘤抑制基因Ｐ５３的改变有着显著的地理差异。应用地高辛标记探针原位杂杂，ＰＣＲ、ＰＣＲ－ＳＳＣＰ分别检测重庆地区高感染、黄曲霉素中等含量３８例ＨＣＣ中ＨＢＶ ＤＮＡ及Ｐ５３的基因突变，并应用Ｓｏｕｔｈｅｒｎ杂交对其中的１２例进行限制性片段多态性分析。

Hepatitis B virus and changes of tumor suppressor gene p53 in hepatocellular carcinoma

The occurrence of the changes of tumor suppressor gene p53 appears to vary with geographic locations.HBV DNA and p53 mutation were observed in 38 cases of hepatocellular carcinoma(HCC)resected in the Chongqing region(a region of high prevalence of HBVand moderate exposure to dietary aflatoxin)with in situ hybridization using digoxigenin labeled probes,PCR and PCR-SSCP analysis.In addition,12cases out of the 38 were studied with Southern blot hybridization to undergo RFLP analysis.It was found that 30 cases out of the 38 were positive for HBV DNA(79%).p53 mutation was found in 9 cases of HCC of which 8 cases were positive for HBV DNA(88.9%,P >0.05).Loss of heterozygosity was demonsrtated only in 2 cases out of 12 HCCcases(16.7%)both of which were positive for HBV DNA (20%,P>0.05).These findings indicate that there is a striking correlation between HBV infection and HCC in the Chongqing region.HBV infection may act synergistically with aflatoxin in the generation of p53 mutation and contributes greatly to the development of HCC.