骨巨细胞瘤组织尿激酶型纤溶酶原激活剂系统mRNA的表达与其生物学行为及复发的关系

目的研究骨巨细胞瘤(giant celltumor of bone,GCT)组织尿激酶型纤溶酶原激活剂(urokinase-type plasminogen activator,u-PA)系统mRNA的表达与GCT病理分级和复发的关系.方法用原位杂交技术检测42例GCT组织u-PA,u-PA受体(u-PAR)及其抑制因子2(PAI-2)mRNA的表达.结果①u-PA,u-PAR,PAI-2 mRNA在GCT组织多核巨细胞(MGC)的阳性率分别为64.3%,71.4%和40.5%;单核基质细胞(MC)的阳性率分别为54.8%,45.2%和9 5%.②Ⅱ、Ⅲ级和复发组GCT的MGCu-PA mRNA与MC u-PA,u-PAR mRNA的表达率均明显高于Ⅰ级(x2分别为8.00,7.82,6.81,P＜0.05)和无复发组(x2分别为5.49,5.54,9.84,P＜0.05);③GCT组织两种细胞间的u-PA mRNA表达(P＜0.05)、MGC u-PAR与PAI-2 mRNA的表达间、MC u-PA与u-PAR mRNA的表达间均呈正相关(x2分别为7.20,8.19,P＜0.05);MGC PAI-2 mRNA的阳性率明显高于MC的阳性率(x2=10.73,P＜0.05).结论GCT组织u-PA,u-PAR mRNA的表达,尤其是在MC中的表达,与骨巨细胞瘤恶性程度密切相关.

Relationship between expression of urokinase-type plasminogen activator mRNA with giant cell tumor of bone and its pathological grading and recurrence

AIM: To investigate the relationship between the expression of uroki- nase-type plasminogen activator(u-PA) mRNA in giant cell tumors of bone(GCT) and pathological grading and recurrence of GCT.METHODS: The expressions of u-PA, u-PA receptor(u-PAR) and u-PA inhibitors(PAI) -2 mRNA in the specimens of GCT obtained by surgical curettement from 42 cases, 13 of grade Ⅰ, 23 of grade Ⅱ, and 6 of grade Ⅲ,and 12 relapsing 5 -32 months later, were detected with in situ hybridization (ISH).RESULTS: ① The expression rates of u-PA, u-PAR, and PAI-2 mRNA in the multinuclear giant cells(MGCs) in the GCT tissues were 64.3%,71.4% and 40.5% respectively, and the expression rates in the mononuclear cells(MCs) were 54.8% , 45.2% and 9.5% respectively. ② The expression rate of u-PA mRNA in MGC, and the expression rates of u-PA and u-PAR mRNAs in MC were significantly higher in the cases of grade Ⅱ and Ⅲ than in the cases of grade I (X2 = 8.00, 7.82, and 6.81, all P ＜0. 05) and significantly higher in the non-recurrent group (X2 = 5.49, 5.54,and 9.84, P＜0.05). ③ A closely positive relationship was shown between the expression of u-PA mRNA in MGC and in MC ( P ＜ 0. 05 ), between the expression of u-PAR and of PAI-2 mRNA in MGC (x2 = 7.20, P ＜ 0. 05),and between the expression of u-PA and expression of u-PAR mRNA in MC (X2 =8.19, P ＜0.05) . The expression rate of PAI-2 mRNA in MGC was higher than that in MC significantly (x2 = 10. 73, P ＜ 0. 05).CONCLUSION: The expressions of u-PA and u-PAR mRNA in GCT tissues, especially in MC, are closely related to the malignancy of GCT.