新型微波探头对组织灭活的实验研究

目的 :验证自行设计的新型微波探头能否增大离体猪肝组织的灭活范围。方法 :设计新型微波探头 ,按不同的条件微波辐射离体猪肝组织 ,A组微波功率为 5 0W ,无盐水灌注 ;B、C、D组微波功率均为 90W ,B组无盐水灌注 ,C组治疗前灌注 5 %盐水 5ml,D组治疗前及治疗同时均灌注 5 %盐水 ,比较其组织灭活范围 ,计算组织灭活体积。结果 :A、B、C、D四组的组织灭活体积分别为 (16 .4 8± 3.86 )cm3、(2 8.86± 6 .15 )cm3、(34.11± 5 .86 )cm3、(40 .5 0± 10 .88)cm3(P值均小于 0 .0 1) ,且D组较C组凝固组织更趋于球形。结论 :本研究设计的新型微波探头在微波功率为 90W ,有气冷循环和盐水灌注双重技术的条件下明显增大了组织的凝固范围 ,且凝固形态更趋于球形 ,可以一次原位灭活较大范围的组织

Experimental Study on Tissue Ablation with Novel Microwave Probe

Objective: To validate a newly designed microwave probe capable of producing larger volume of ablated lesions in ex vivo swine livers. Methods: A novel microwave probe was developed and tested in ex vivo specimens of swine liver. Group A was not given saline infusion under power output 50 W. Group B, C, D under power output 90 W were tested respectively according to the condition of no saline infusion ( group B), 5 min preinfusion ( 5 mlsaline, group C), preinfusion and continuous infusion (at the rate of 1 ml/rmin, group D) using the novel probe. The sizes of the microwave-induced lesions were evaluated and the volume of the tissue destruction qualified. Results: The approximate volumes of the microwave-induced lesions were ( 16.48 ± 3.86) cm3, (28.86 ± 6.15) cm3, (34.11 ± 5.86)cm3, (40.50 ± 10.88) cm3, respectively ( P ＜ 0.01). Preinfusion and continuous infusion (group D) created more spherical lesions than only preinfusion (group C). Conclusion: The newly-developed microwave probe increased the volume of coagulation necrosis, created a more spherical lesion under the power output of 90 W, and might achieve the effective treatment of larger neoplasm with a single application of microwave energy.