大鼠输尿管梗阻后肾炎中的P-选择素表达及意义

目的：探讨粘附分子Ｐ－选择素在单侧输尿管梗阻后肾炎中的作用。方法建立单侧输尿管梗阻（ＵＵＯ）动物模型，观察肾组织病理改变和Ｐ－选择素在肾组织中的表达，以及抗Ｐ－选择素单克隆抗体（单抗）对其。结果大鼠ＵＵＯ第１ｄ即见间质以单核巨噬细胞为主的炎细胞浸润和Ｐ－选择素在小管间质表达；第３ｄ间质炎细胞增多，Ｐ－选择素明显表达，第７ｄ间质炎细胞浸润达高峰伴间质水肿，而Ｐ－选择表达下降，抗Ｐ－选择素单抗治疗组炎

Significance of P- selectin Expression in Unilateral Ureteral Obstruction

Objective To determine the relationship between P-selectin and unilateral ureteral obstruction (UUO). Methods The effect of anti-P-selectin monoclonal antibody (P-selectin mAb) on the renal histopathological changes and P-selectin expression was investigated in the UUO experimental model. Results Renal interstitial macrophage infiltration was observed within 24h after UUO, with marked increase at day 3 and a peak response at day 7. Interstitial edema was also found at day 7. The expressin of P-selectin was upregulated on the tubular epithelium and within the interstitium as early as 24h after ureteral ligation, with a peak expression at day 3 and down-regulated at day 7. Macrophage accumulation was significantly inhibited when P-selectin was blocked by mAb. Conclusion P-selectin plays a role in interstitial macrophage infiltration in the early phase of UUO, and P-selectin mAb might improve the interstitial leisons.