注射用丹参多酚酸对大鼠脑缺血再灌注线粒体ATP酶活性的影响

目的探讨注射用丹参多酚酸对大鼠脑缺血再灌注线粒体ATP酶活性的影响。方法将54只雄性SD大鼠随机分成3组(n=18):假手术组(Sham组)、缺血再灌注组(IR组)、丹参多酚酸组(Salvianolate组)。HE染色法观察大脑神经元的组织病理学变化;TTC染色检测脑梗死体积;分光光度计发法线粒体丙二醛含量和线粒体Na~+/K~+ATP酶、Ca~(2+)ATP酶、Mg~(2+)ATP酶活性。结果假手术组大鼠脑组织红染,未见梗死灶;丹参多酚酸组神经细胞变性坏死程度、脑梗死面积较缺血再灌注组明显减轻;与缺血再灌注组相比,丹参多酚酸组线粒体丙二醛含量下降(P0.05)和线粒体Na~+/K~+ATP酶、Ca~(2+)ATP酶、Mg~(2+)ATP酶活性升高(P0.05)。结论注射用丹参多酚酸对缺血再灌注损伤有保护作用,其机制与提高线粒体ATP酶活性有关。

Effect of salvianolate injection on ATPase activity of mitochondria in rats with cerebral ischemia reperfusion

Objective To explore the effect of salvianolate injection on ATPase activity of mitochondria in rats with cerebral ischemia reperfusion.Methods Totally 54 male Sprague Dawley(SD)rats were randomly divided into three groups:sham-operation group,ischemia-reperfusion(I/R)group and salvianolate group,eighteen rats in each group.Histopathological changes of cerebral neurons were observed by HE staining,volume of cerebral infarction were measured by staining with triphenyltetrazolium chloride(TTC)and activities of Na+/K+ ATPase,Ca2+ ATPase and Mg2+ ATPase of mitochondria were measured by spectrophotometer.Results The sham group showed red cerebral tissues,which indicated no infarction.Compared with those in ischemia-reperfusion group,degeneration degree of neurons and volume of cerebral infarction were significantly reduced,malondialdehyde(MDA)content was decreased and the activities of Na+/K+ ATPase,Ca2+ ATPase and Mg2+ ATPase of mitochondria in salvianolate group were increased(all P<0.05).Conclusion Salvianolate has a neuroprotective effect on focal cerebral ischemia reperfusion in rats,whose mechanism may be associated with increased ATPase activity of mitochondria.