耐力训练和一次性力竭运动对大鼠心肌和骨骼肌硫氧还蛋白还原酶的影响

目的:观察运动对大鼠心肌、骨骼肌硫氧还蛋白还原酶(TR)的影响.方法:SD大鼠30只,随机分成安静对照组、耐力训练组和力竭组.训练组进行6周渐增游泳训练,5次/周.最后1次训练后24小时,断头处死安静对照组和训练组大鼠,力竭组大鼠在一次性力竭运动后即刻处死.DTNB法检测心肌、骨骼肌TR活性,RT-PCR法观察TR mRNA水平变化.结果:6周耐力训练大鼠心肌TR活性显著高于安静对照组,一次性力竭组大鼠心肌TR活性显著低于安静对照组.各组大鼠心肌TR mRNA均无显著性差异.结论:6周耐力训练显著提高大鼠心肌TR活性,而在mRNA水平上无显著性差异.

The Influences of Endurance Training and Single Bout of Exhaustive Exercise on Thioredoxin Reductase of Myocardium and Skeletal Muscle in Rats

Objective The present study aimed at investigating the influences of exercise on thioredoxin reductase(TR)of myocardium and skeletal muscle in rats. Methods Thirty SD rats were randomly divided into 3 groups: sedentary control group(CG), endurance training group(TG) and exhaustive exercise group(EG). The rats in TG performed incremental loading swimming, 5 days per week for 6 weeks, and then were sacrificed within 24 hours after the last swimming. The rats in EG were sacrificed immediately after the exhaustive swimming. The activity and the mRNA of thioredoxin reductase were determined respectively by DTNB and RT-PCR. Results (1)6 weeks after training, the activity of thioredoxin reductase in myocardium of TG was significantly increased, compared with that of CG and EG. In skeletal muscle, statistical significance was only observed between TG and EG. (2) There was no difference in mRNA level in myocardium among three groups. Conclusion Prolonged exercise or aerobic exercise affected significantly the activity of thioredoxin reductase without influence on mRNA.