| 不育男性精子染色质结构与精液常规参数的关系 |
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| 引用本文： | 谷龙杰,陈振文,许剑锋. 不育男性精子染色质结构与精液常规参数的关系[J]. 中国男科学杂志, 2009, 23(3) |
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| 作者姓名： | 谷龙杰 陈振文 许剑锋 |
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| 作者单位： | 1. 国家人口计生委科学技术研究所,北京,100081;北京协和医学院研究生院
2. 国家人口计生委科学技术研究所,北京,100081 |
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| 摘 要： | 目的 研究不育男性精子染色质结构参数与精液常规参数的关系.方法 采集36例男性不育患者和18例健康对照者的精液标本,用TUNEL法检测精子DNA碎片化,CMA3染色法检测精子染色质包装质量,按照世界卫生组织标准(1999)检测精液常规参数.结果 不育组的精子TUNEL阳性率和CMA3阳性率均显著高于健康对照组,差异具有统计学意义(P＜0.05),精液常规参数正常的不育男性精子TUNEL阳性率和CMA3阳性率也显著高于健康对照组,差异具有统计学意义(P＜0.05),精子TUNEL阳性率和CMA3阳性率与精液常规参数之间具有显著的相关性(P＜0.05).结论 不育男性的精子染色质结构检测结果与健康男性存在显著差异,精子染色质结构检测可提供反映男性生育能力的信息.
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| 关 键 词： | 不育 男性 精子 染色质 精液 |
Relationship between sperm chromatin structure and semen parameters of infertile men |
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| Gu Longjie,Chen Zhenwen,Xu Jianfeng. Relationship between sperm chromatin structure and semen parameters of infertile men[J]. Chinese Journal of Andrology, 2009, 23(3) |
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| Authors: | Gu Longjie Chen Zhenwen Xu Jianfeng |
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| Abstract: | Objective To analyze the relationship between sperm chromatin structure and semen conventional parameters of infertility men.Methods Semen samples were collected from thirty-six infertility male patients and eighteen healthy donors.Sperm DNA fragmentation was measured with TUNEL and sperm chromatin packaging was measured with CMA3 staining.Semen conventional analysis was performed based on WHO criteria(1999).Results Positive rates of TUNEL and CMA3 staining in the infertility group were significantly higher... |
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| Keywords: | infertility male spermatozoa chromatin semen |
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