DNA hydroxymethylation rate in the AChE and HoxC4 promoter associated with human sperm quality |
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| Authors: | Y Li Y H Li X Zhou B Wu J P Chen Z K Wang X Wang H J Shi R S Li |
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| Institution: | 1. School of Pharmacy, Fudan University, Shanghai, China;2. Key Laboratory of Reproduction Regulation of NPFPC, SIPPR, IRD, Fudan University, Shanghai, China;3. The hospital affiliated to Shanghai Institute of Planned Parenthood Research, Shanghai, China |
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| Abstract: | The relationship of altered DNA 5′‐hydroxymethylation in human spermatozoa with seminal parameters remains unclear. The aim of the study was to investigate the association between the 5′‐hydroxymethylcytosine (5hmC) rate in the promoters of acetylcholinesterase (AChE) and homeobox C4 (HoxC4) genes and human sperm concentration/motility. The study population consisted of three groups: asthenozoospermia (AZ), oligoasthenozoospermia (OAZ) and normozoospermia (NZ). The 5hmC rate in the promoter was measured by CCGG loci‐dependent MspI/HpaII restriction mapping of glycosylation‐modified sperm DNA combined with a hydroxymethylation‐specific real‐time polymerase chain reaction assay. The 5hmC rate in the AChE promoter in group AZ and OAZ was higher than that in group NZ (p < .05). A weak inverse correlation between 5hmC rate of AChE and sperm motility was observed in all subjects (r = ?.172, p < .05). The 5hmC rate in the HoxC4 promoter in group OAZ was lower than that in group NZ (p < .05). These results indicated that altered 5hmC rates of AChE and HoxC4 promoters are associated with low sperm motility and sperm concentration respectively. |
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| Keywords: | 5‐hydroxymethylcytosine AChE DNA hydroxymethylation HoxC4 sperm motility |
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