首页 | 本学科首页   官方微博 | 高级检索  
检索        


Chlorogenic acid improves the quality of boar semen subjected to cooled storage at 15°C
Authors:B A Pereira  B R Chaves  M C Teles  T P Pontelo  C R Oliveira  R V de Souza  J E Rodríguez‐Gil  M G Zangeronimo
Institution:1. Department of Veterinary Medicine, Federal University of Lavras, Lavras, Brazil;2. Department of Animal Medicine and Surgery, Autonomous University of Barcelona, Bellaterra (Cerdanyola del Vallès), Spain
Abstract:The aim of this study was to investigate the effect of chlorogenic acid (ChA) in boar semen stored at 15°C. Twelve ejaculates were processed into insemination doses at different concentrations of ChA (0.0, 1.5, 3.0, 4.0 and 6.0 mg/ml) or vitamin E (200 μl/ml) as positive control. Semen was analysed after 0, 24 and 72 hr of storage. ChA improved (p < .05) sperm motility, acrosome integrity and mitochondrial activity in all periods of storage. Furthermore, after 24 hr of storage, ChA above 1.5 mg/ml supported the sperm viability until 120 min after reheating (p < .05). Both ChA and vitamin E were similarly efficient in increasing the antioxidant capacity of semen, reducing the malondialdehyde levels before and after 72 hr of storage (p < .05). However, with 72 hr of storage, ChA at 3.0 mg/ml improved the mitochondrial activity over vitamin E (p < .05). In conclusion, results suggest that the concentration of 3.2 mg/ml of ChA is the best for semen stored for up to 24 hr. However, for semen stored for a longer period, 6.0 mg/ml or more should be used.
Keywords:antioxidant  boar spermatozoa  chlorogenic acid  oxidative damage  semen storage
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号