内皮抑素抑制小鼠前胃癌细胞(MFC)的探讨

目的 :研究人内皮抑素对裸小鼠前胃癌 (MFC)生长和转移的抑制作用。方法 :建立 MFC小鼠肿瘤动物模型。将 72只荷瘤小鼠随机分成 4组 ,对照组及治疗各组 ,每组均为 18只 ,治疗组分小、中、大剂量组。小剂量组皮下注射内皮抑素 ,浓度为 5 m g/ kg,中剂量组皮下注射内皮抑素 ,浓度为 10 mg/ kg,大剂量组皮下注射内皮抑素 ,浓度为 15 mg/ Kg,对照组注射等量无菌生理盐水。注射次数均为每两天 1次 ,共 6周。第 7周处死小鼠 ,测量原位肿瘤的直径、体积 ,计算抑瘤率 ;测定肿瘤细胞的增生指数及凋亡指数。结果 :内皮抑素治疗组 (小、中、大剂量 )能明显抑制肿瘤的生长 ,与对照组比较有显著性的差异(P<0 .0 5 ) ;内皮抑素治疗组能降低肿瘤微血管密度 ,抑制肿瘤血管的形成 ,与对照组相比有显著性差异 ;治疗组肿瘤细胞凋亡指数明显上升 ,优于对照组 (P <0 .0 5 )。结论 :内皮抑素通过抗肿瘤血管生成 ,促进肿瘤细胞凋亡 ,对肿瘤的生长和转移均有较强的抑制作用。

Inhibition of MFC tumor cell implanted in nude mice by endostatin

Objective: To study of the inhibition of growth of MFC tumor cell implanted in nude mice by endostatin. Methods:We established the tumor model in nude mice with MFC cell,then divided these mice into four groups, control group,little dose ,middle dose and large dose groups.Brine was injected in the control group every other day,and different doses of endostatin was injected in other groups every other day.After seven weeks,we killed the mice,measured the diameter and the volume of the tumor,the apoptotic index and proliferative index of the tumor cell.Results: Endostatin restrained the growth of tumor and the vein of tumor,with notable differences between treatment groups and control group.And the apoptotic index rose clearly in the treatment groups.Conclusions: Endostatin can restrain the vein of the tumor,accelerate the apoptotic of tumor cell,inhibit the growth of tumor in nude mice.