碱性成纤维生长因子的表达对大鼠视神经损伤修复的意义

目的:观察碱性成纤维生长因子(bFGF)在视神经中的表达及在视神经损伤后的反应和作用。方法:健康Wistar大鼠20只,雌雄不拘,按处理方式随机分为3组,视神经钳夹伤8只(A组),假伤对照组8只(B组),正常对照4只(C组)。采用大鼠球后视神经钳夹伤模型,利用免疫组化法和计算机图像分析技术,于术后1,3,7,14d检测视神经bFGF的表达。结果:损伤后胶质细胞排列紊乱,胞体增大。视神经损伤后(1,3,7,14d)bFGF阳性细胞表面积密度(%)分别为2.20±0.18,2.18±0.17,2.29±0.45,2.32±0.37,与假伤组(1.52±0.33,1.51±0.37,1.50±0.42,1.48±0.32)和对照组(1.49±0.29,1.50±0.30,1.49±0.35,1.51±0.29)比较,差异有显著性意义(t=3.037～4.158,3.531～4.182,P<0.05)。结论:视神经损伤提高bFGF在视神经中的表达,对视神经损伤的修复具有积极的作用。

Expression of basic fibroblast growth factor in repair of injured rat optic nerve

AIM:To investigate changes in the expression of basic fibroblast growth factor (bFGF) in rat optic nerve after injury. METHODS:Twenty normal Wistar rats were randomly divided into 3 groups to receive optic nerve injury(group A,n=8),sham optic nerve injury (group B,n=8) or no treatment (group C,n=4).Intraorbital optic nerve injury models were established and bFGF expression was determined immunohistochemically at 1, 3, 7, and 14 days respectively after injury,with the results examined by automated image analysis system. RESULTS:The glial cells were enlarged and confusingly arranged following optic nerve injury,and surface density of bFGF positive cells(% ) in group A was (2.20±0.18)% ,(2.18±0.17)% ,(2.29±0.45)% ,(2.32± 0.37)% ,respectively,in comparison with group B (1.52±0.33)% ,(1.51±0.37)% ,(1.50±0.42)% ,(1.48±0.32)% ] and group C(1.49±0.29)% ,(1.50±0.30)% ,(1.49±0.35)% ,(1.51±0.29)% ] (t=3.037-4.158, 3.531-4.182, P<0.05),there was a significant difference between three groups. CONCLUSION:Optic nerve injury gives rise to elevated expression of bFGF in the optic nerve,which can be beneficial for the repair of the nerve injury.