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| 低强度激光照射对大鼠血管平滑肌细胞一氧化氮生成通路的上调作用 | |
| 引用本文： | 钟光珍,崔鸣,陈凤荣,赵晶,唐朝枢. 低强度激光照射对大鼠血管平滑肌细胞一氧化氮生成通路的上调作用[J]. 中国激光医学杂志, 2008, 17(4) |
| 作者姓名： | 钟光珍 崔鸣 陈凤荣 赵晶 唐朝枢 |
| 作者单位： | 1. 首都医科大学附属北京朝阳医院心脏中心,北京市,100020 2. 北京大学第三医院心内科 3. 北京大学生理与病理生理教研室 |
| 基金项目： | 国家自然科学基金，北京市自然科学基金 |
| 摘 要： | 目的 观察低强度激光照射(low power laser irradiation,LPLI)对大鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)左旋精氨酸(L-Arg)转运、一氧化氮(NO)生成以及NO合酶(nitric oxide synthase,NOS)活性的作用,以探讨LPLI血管保护作用的细胞分子机制.方法 取Wistar大鼠主动脉,贴块法培养VSMCs,分别以能量密度0、0.9.2.7和4.5 J/cm2的He-Ne激光(波长632nm)照射,无照射组为对照组.重氮化学反应法测定培养液中NO含量,细胞孵育液中加入3H-L-Arg测定细胞L-Arg的摄入,测定3H-胍氨酸的生成反映NOS活性.结果 能量密度分别为0.9,2.7和4.5 J/cm2的LPLI照射VSMCs,促进细胞NO生成分别较对照组增加-1.3%(P＞0.05)、60.4%(P＜0.01)和78.6%(P＜0.01);细胞NOS活性分别较对照组增加16.9%(P＞0.05)、44.6%(P＜0.01)和53.0%(P＜0.01);而且细胞摄入L-Arg(Vmax值)分别较对照组增加11.0%(P＜0.05),66.7%(P＜0.01)和38.7%(P＜0.01);激光照射对L-Arg跨细胞膜转运的米氏常数(Km值)无明显影响(均P＞0.05).结论 低强度He-Ne激光照射激活大鼠VSMCs的L-Arg/NOS/NO通路,可能为低强度激光扩张血管、改善器官血流等生物学效应的机制之一. |
| 关 键 词： | 低强度激光 一氧化氮 血管平滑肌细胞 |
Low Power Laser Irradiation Upregulates L-Arginine/Nitric Oxide Pathway in Cultured Rat Vascular Smooth Muscle Cells |
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| ZHONG Guang-zhen,CUI Ming,CHEN Feng-rong,ZHAO Jing,TANG Chao-shu. Low Power Laser Irradiation Upregulates L-Arginine/Nitric Oxide Pathway in Cultured Rat Vascular Smooth Muscle Cells[J]. Chinese Journal of Laser Medicine & Surgery, 2008, 17(4) | |
| Authors: | ZHONG Guang-zhen CUI Ming CHEN Feng-rong ZHAO Jing TANG Chao-shu |
| Affiliation: | ZHONG Guang-zhen1,CUI Ming2*,CHEN Feng-rong2,ZHAO Jing3,TANG Chao-shu3 1.Department of Cardiology,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020 China 2.Department of Cardiology,Peking University the Third Hospital,Beijing 3.Department of Physiology , Pathophysiology,Peking University,Beijing |
| Abstract: | Objective To investigate the cellular and molecular mechanism of low power laser irradiation(LPLI) by studying the effects of LPLI on nitric oxide(NO) production, L-arginine(L-Arg) uptake and NO synthase(NOS) activity in cultured rat vascular smooth muscle cells(VSMCs). Methods Rat VSMCs were obtained by explantation from rat aorta. VSMCs were cultured with Dulbecco's modified Eagle's medium containing 10% fetal bovine serum. VSMCs were irradiated with low power red laser (632 nm) at doses of 0.9, 2.7 and 4... |
| Keywords: | Low power laser irradiation Nitric oxide Vascular smooth muscle cells |
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