日本血吸虫混合DNA疫苗pBK-CMV-Sj26/Sj32诱导小鼠的免疫应答

目的　观察含日本血吸虫候选疫苗分子谷胱甘肽S -转移酶 (Sj2 6 )和天冬酰胺肽链酶 (Sj32 )的真核载体 pBK-CMV -Sj2 6 /Sj32诱导小鼠的免疫应答情况。 方法　将质粒按 5 0 μg/只在 0w ,3w ,5w免疫小鼠股四头肌 ,第一次免疫后取小鼠脾细胞作T淋巴细胞转化实验 ;在 0w、3w和 6w经小鼠尾静脉取血 ,ELISA检测血清中抗体效价及阳性反应率。结果　淋巴细胞转化实验示血吸虫成虫抗原能特异性刺激免疫鼠脾细胞 ;第一次免疫后特异性抗体效价开始升高。抗体最高效价为 1∶2 0 ,3次免疫后有 91.7%小鼠血清呈阳性反应。结论　真核载体 pBK -CMV -Sj2 6 /Sj32可诱导小鼠体液及细胞免疫应答

Immune response of mixture of DNA vaccine pBK-CMV-Sj26/Sj32 to Schistosoma japonicum in inoculated mice

Aim To observe immune responses to Schistosoma japonicum in mice induced by DNA immunization with plasmid pBK-CMV-Sj26/Sj32.Methods Each mouse was inoculated with the DNA vaccine pBK-CMV-Sj26/Sj32 into quadriceps at the weeks of 0,3,5.T lymphcyte transformation test was adopted to confirm the cellular immune responses to S.japonicum after the first inoculation.The sera were collected from tail vein of mice at the weeks of 0,3,6 and then ELISA was used to confirm the humoral immune responses. Results Lymphocyte transformation test showed that the specific proliferated response of spleen cells from immunized mice was remarkably higher than that of control group.Compared with the control group,the titre of the immune serum got high and the positive rate of immunized group got 91.7% after the third injection.Conclusion The humoral and cellular immune responses could be induced in mice by naked DNA pBK-CMV-Sj26/Sj32 injection.