分光光度法测定不同产地款冬花的总黄酮含量

目的：建立款冬花中黄酮类化合物的提取方法和含量测定方法,并比较10个不同产地款冬花中总黄酮的含量。方法：以芦丁为对照品,NaNO2-Al（NO3）3-NaOH为显色剂,在520 nm处测定款冬花中总黄酮的含量。结果：在7.8～46.8 mg/L范围内,吸光度与浓度线性关系良好（r=0.999 7）,平均回收率为98.04%（RSD=1.00%）,不同产地款冬花总黄酮含量为4.2%～8.0%。结论：不同产地款冬花药材中黄酮类化合物含量存在较大差异。

Determination of Flavonoids in Tussilago Farfara L. from Different Areas by Spectrophotometry

Objective： To develop a method for determination of flavonoids in Tussilago farfara from different areas.Methods： The flavonoids in Tussilago farfara were determined by spectrophotometry at 520 nm.Rutin was used as standard for comparison.The color reagent was a mixture of NaNO2-Al（NO3）3-NaOH solution.Results： The calibration curve was linear in the range of 7.8～46.8 mg/L（r=0.999 7）.The average recovery was 98.04%（RSD = 1.00%）.The contents of flavonoids in Tussilago farfara from different areas were 4.2%～8.0%.Conclusions： The yields of flavonoids in Tussilago farfara from different areas are different.