妊娠相关血浆蛋白-A在兔动脉粥样硬化斑块中的差异表达

目的：研究妊娠相关血浆蛋白-A（PAPP-A）在兔动脉粥样硬化易损斑块模型中胸主动脉中的表达差异。方法：建立兔动脉粥样硬化模型,然后给予中国斑点蝰蛇毒（CRVV）和组胺药物触发,造成实验性斑块破裂和血栓形成。细胞免疫组织化学法检测PAPP-A的表达,并确定表达部位。结果：免疫组织化学在胸主动脉粥样破裂斑块中,PAPP-A染色强阳性;不稳定斑块PAPP-A染色为阳性;而稳定斑块、正常组织中均无PAPP-A表达。PAPP-A在易损斑块的表达主要集中血管平滑肌细胞、内皮细胞、巨噬细胞中表达。结论：PAPP-A在构建的兔动脉粥样硬化易损斑块中表达,为进一步的研究PAPP-A在ACS中的作用机制奠定试验基础。

Differential expression and significance of PAPP-A in the model artherosclerosis unstable plaque

Objective： To study the expression and significance of （Pregnancy-Associated plasma protein A PAPP-A） in the model about artherosclerosis unstable plaque. Method：The rabbit model of atherosclerosis vulner- able plaque were constructed, and then they were to be cause （induced） to happen rupture and thrombosis of plaque through CRVV and histamine. The tissue immunohistochemistry method was used in order to detect and make sure the position for protein expression of PAPP-A. Result： The immunohistochemistral results showed that the expression of PAPP-A in vulnerable plaques were strong positive and which located in the cytoplasm. And the expression of PAPP-A in stable plaques were feeble. But there was no PAPP-A expressed in the normal aorta tis- sues. Conclusion：There was a significant difference in the expression of PAPP-A in different pathology plaques and tissues. It was mostly seen from the vulnerable plaques and located in the cytoplasma.