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| 红芪多糖的脱蛋白及脱色素工艺 | |
| 引用本文： | 李向东,惠和平,封士兰,欧阳晓玫.红芪多糖的脱蛋白及脱色素工艺[J].中国当代医药,2010,17(3):40-41. |
| 作者姓名： | 李向东 惠和平 封士兰 欧阳晓玫 |
| 作者单位： | 1. 甘肃省嘉峪关市血站,甘肃嘉峪关,735100 2. 兰州大学药学院,甘肃兰州,730000 3. 甘肃省药品检验所,甘肃兰州,730000 |
| 基金项目： | 甘肃省科技计划项，兰州市科技计划项目 |
| 摘 要： | 目的：研究红芪多糖（HPS）的脱蛋白、脱色工艺。方法：采用L9（34）正交试验和单因素试验分别优选Sevage法脱蛋白工艺与过氧化氢脱色素工艺。结果：Sevage法脱蛋白工艺为：样液与试剂的体积比为1∶1,氯仿与正丁醇的体积比为4∶1,振摇30min,静置20min。过氧化氢脱色素条件是脱色时间为2～4h,用量为5～20ml过氧化氢每50ml样液。结论：优化后的条件适合于红芪多糖的脱蛋白、脱色素。 |
| 关 键 词： | 红芪多糖 脱蛋白 脱色素 |
The process of removing proteins and depigmentation of polysaccharide from Radix Hedysari |
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| Authors: | LI Xiangdong HUI Heping FENG Shilan OUYANG Xiaomei |
| Institution: | LI Xiangdong, HUI Heping, FENG Shilan, OUYANG Xiaomei (1.Jiayuguan Blood Center of Gansu Province, Jiayuguan 735100,China;2.School of Pharmacy, Lanzhou University, Lanzhou 730000, China;3.Gansu Institute for Drug Control,Lanzhou 730000,China) |
| Abstract: | Objective： To study the process of removing proteins and depigmentation of polysaccharide from Radix Hedysari. Methods： Used L9（34） orthogonal test and single factor experiment optimize the technological of removing proteins by Sevag and depigmentation by H2O2. Results： The optimal technical conditions of deproteinization of Sevag were： the volume ratio of polysaccharides solution-Sevag reagent was 1：1,the volume ratio of chloroform-n-butanol was 4：1,shaked for 30 minutes,and put for 20 minutes. The process of depigmentation of H2O2 was： the time of depigmentation was 2 to 4 h, the adding amount of H2O2 was 5 to 20 ml per 50 ml sample solution. Conclusion： The optimal process of removing proteins and depigmentation is fit for polysaccharide from Radix Hedysari. |
| Keywords: | Radix Hedysari Polysaccharide Removal of protein Depigmentation |
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